PXD008520

PXD008520 is an original dataset announced via ProteomeXchange.

Title	Distinct branches of the N-end rule pathway modulate the plant immune response
Description	The N-end rule pathway is a highly conserved constituent of the ubiquitin proteasome system, though little information exists regarding biological roles. Here we show that the cysteine (Cys-) and glutamine (Gln)-specific amino-terminal (Nt)-amidase NTAQ1 branches are both components of the plant immune system, through the E3 ligase PROTEOLYSIS (PRT)6. Both ntaq1 and prt6 mutants of Arabidopsis thaliana showed enhanced basal resistance to the hemibiotroph pathogen Pseudomonas syringae, associated with constitutive expression of defence-response genes and activation of synthesis pathway of the phytoalexin camalexin. We observed a role in stomatal defence for the Nt-Cys substrates ETHYLENE RESPONSE FACTOR VII transcription factors. Transgenic barley lines with reduced HvPRT6 expression showed enhanced resistance against Ps japonica and Blumeria graminis f. sp. Hordei, indicating a conserved role of the pathway in immunity. These data demonstrate that different branches of the N-end rule pathway act at distinct levels of the plant immune response.
HostingRepository	PRIDE
AnnounceDate	2018-11-06
AnnouncementXML	Submission_2018-11-06_08:22:01.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jarne Pauwels
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-20 07:37:43	ID requested
⏵ 1	2018-11-06 08:22:02	announced

Vicente J, Mendiondo GM, Pauwels J, Pastor V, Izquierdo Y, Naumann C, Movahedi M, Rooney D, Gibbs DJ, Smart K, Bachmair A, Gray JE, Dissmeyer N, Castresana C, Ray RV, Gevaert K, Holdsworth MJ, Distinct branches of the N-end rule pathway modulate the plant immune response. New Phytol, 221(2):988-1000(2019) [pubmed]

curator keyword: Biological

submitter keyword: Plant immunity, N-end rule pathway, Amino-terminal glutamine amidase, NTAQ, ERFVII

Michael J. Holdsworth
contact affiliation	School of Biosciences, University of Nottingham, LE12 5RD, UK
contact email	Michael.Holdsworth@nottingham.ac.uk
lab head
Jarne Pauwels
contact affiliation	VIB-Ugent
contact email	jarne.pauwels@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD008520

PRIDE project URI

• PRIDE
  1. PXD008520
     1. Label: PRIDE project
     2. Name: Distinct branches of the N-end rule pathway modulate the plant immune response