PXD008486

PXD008486 is an original dataset announced via ProteomeXchange.

Title	Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting
Description	Ahead of display, a non-original layer was observed on the surface of a fragment of a wall painting by Ambrogio Lorenzetti (active 1319, died 1348/9). FTIR analysis suggested proteinaceous content. Mass spectrometry was used to better characterise this layer and revealed two protein components: sheep and cow glue and chicken and mallard egg white. Analysis of posttranslational modifications detected several photo-oxidation products, which suggest that the egg experienced prolonged exposure to UV-light and was likely applied long before the addition of the glue. Additionally, glycation products detected may indicate naturally occurring glycoprotein degradation or reaction with a carbohydrate material such as starch, identified by ATR-FTIR in a cross-section of a sample taken from the painting. Palaeoproteomics is shown to provide detailed characterisation of organic layers associated with mural paintings and therefore aids reconstruction of the conservation history of these objects.
HostingRepository	PRIDE
AnnounceDate	2018-04-05
AnnouncementXML	Submission_2018-04-05_04:53:00.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Meaghan Mackie
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Gallus gallus (Chicken); NCBI TaxID: 9031; scientific name: Anas platyrhynchos (Domestic duck) (Anas boschas); NCBI TaxID: 8839; scientific name: Ovis aries (Sheep); NCBI TaxID: 9940;
ModificationList	dihydroxylated residue; monohydroxylated residue; deamidated residue; glycosylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-18 09:03:42	ID requested
⏵ 1	2018-04-05 04:53:01	announced

Mackie M, R, ü, ther P, Samodova D, Di Gianvincenzo F, Granzotto C, Lyon D, Peggie DA, Howard H, Harrison L, Jensen LJ, Olsen JV, Cappellini E, Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting. Angew Chem Int Ed Engl, 57(25):7369-7374(2018) [pubmed]

curator keyword: Technical

submitter keyword: conservation science, cultural heritage, mass spectrometry, posttranslational modifications, proteomics

Enrico Cappellini
contact affiliation	Natural History Museum of Denmark University of Copenhagen, Øster Voldgade 5–7, 1350 Copenhagen, Denmark
contact email	ecappellini@snm.ku.dk
lab head
Meaghan Mackie
contact affiliation	University of Copenhagen
contact email	meaghan@palaeome.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008486

PRIDE project URI

• PRIDE
  1. PXD008486
     1. Label: PRIDE project
     2. Name: Palaeoproteomic Profiling of Conservation Layers on a 14th Century Italian Wall Painting