PXD008483

PXD008483 is an original dataset announced via ProteomeXchange.

Title	Crotonylome profiling reveals extensive lysine crotonylation in common wheat
Description	Histone lysine crotonylation (Kcr) is a newly discovered protein post-translational modification (PTM), which was detected from yeast to humans and is mainly related with active transcription. With the development of proteomics technologies, high abundance of non-histone proteins modified by Kcr was also found recently. Here, we first report that lysine Kcr also occurs on cytoplasmic and mitochondrial proteins in common wheat (Triticum aestivum L.). We identified 4,696 Lys-acetylated sites on 1,726 proteins which involved in a wide variety of biological processes, such as chromatin-associated processes, Calvin-Benson cycle, glycolysis, protein metabolism and transport, which representing the largest dataset of lysine acylation proteome reported in the plant kingdom. Interestingly, 98 proteins were involved in multiple processes of photosynthesis, suggesting an important role of lysine Kcr in processes. In addition, 21 potentially specific Kcr motifs in wheat were detected. The protein interaction network analysis revealed that diverse interactions are modulated by protein Kcr. The overlap between Kcr and acetylation (Kac) indicated that they may coordinately regulate the function of some proteins in common wheat. Futhermore, comparative analysis indicated that lysine Kcr is conserved between common wheat and Nicotiana tabacum. Taken together, this study provided the first global survey of Kcr in wheat, making a promising starting point for further functional analysis of Kcr in plants.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_09:02:16.372.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	suzhen huang
SpeciesList	scientific name: Triticum aestivum (Wheat); NCBI TaxID: 4565;
ModificationList	N6-crotonyl-L-lysine
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-18 03:27:08	ID requested
1	2023-07-20 10:34:35	announced
⏵ 2	2023-11-14 09:02:20	announced	2023-11-14: Updated project metadata.

Zhang N, Wang S, Zhao S, Chen D, Tian H, Li J, Zhang L, Li S, Liu L, Shi C, Yu X, Ren Y, Chen F, model regulating wheat cold tolerance through mediating pyruvate. Sci Adv, 9(19):eadg1012(2023) [pubmed]

curator keyword: Biological

submitter keyword: Triticum aestivum L.

post-translational modification

lysine crotonylation

proteomics

mass spectrometry

Feng Chen
contact affiliation	Agronomy College, Henan Agricultural University, China
contact email	chf0088@163.com
lab head
suzhen huang
contact affiliation	PTM
contact email	13920817975@163.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008483
     1. Label: PRIDE project
     2. Name: Crotonylome profiling reveals extensive lysine crotonylation in common wheat