PXD008478

PXD008478 is an original dataset announced via ProteomeXchange.

Title	Shotgun proteomic profiling of spleen of rainbow trout Oncorhynchus mykiss
Description	The objective of this study was to identify and quantify proteomic profiles of spleen of rainbow trout Oncorhynchus mykiss. Specific pathogen free rainbow trout (mean length 15 ± 1 cm) were maintained in recirculating de-chlorinated water at 19±1 °C. Prior to the experiment, fish were distributed between 9 aquaria, 18 fish per aquarium. The test groups were infected by immersion of Yersinia ruckeri strains: CSF007-82 (biotype 1) and 7959-11 (biotype 2). The control group was immersed similar with sterile broth medium. There were 3 aquaria per each group (CSF007-82-infected, 7959-11-infected and control). Nine fish from infected and control fish groups were anaesthetized with MS-222 at 3, 9 and 28 days post exposure and sampled aseptically. Each spleen was washed three times with sterile phosphate-buffered saline containing a cocktail of mammalian protease inhibitors. Spleen samples were snap-frozen in liquid nitrogen and stored at –80 °C.
HostingRepository	PRIDE
AnnounceDate	2018-08-14
AnnouncementXML	Submission_2018-09-24_00:17:25.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gokhlesh Kumar
SpeciesList	scientific name: Oncorhynchus mykiss (Rainbow trout) (Salmo gairdneri); NCBI TaxID: 8022;
ModificationList	iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-18 00:28:56	ID requested
1	2018-08-14 04:00:30	announced
⏵ 2	2018-09-24 00:17:27	announced	Updated publication reference for PubMed record(s): 30094954, 30228307.

Kumar G, Hummel K, Noebauer K, Welch TJ, Razzazi-Fazeli E, El-Matbouli M, Proteome analysis reveals a role of rainbow trout lymphoid organs during Yersinia ruckeri infection process. Sci Rep, 8(1):13998(2018) [pubmed]

Kumar G, Hummel K, Razzazi-Fazeli E, El-Matbouli M, Proteome Profiles of Head Kidney and Spleen of Rainbow Trout (Oncorhynchus Mykiss). Proteomics, 18(17):e1800101(2018) [pubmed]

curator keyword: Biological

submitter keyword: Rainbow trout, spleen, LC MS-MS, Proteome profile

Dr. Gokhlesh Kumar
contact affiliation	Clinical Division of Fish Medicine, University of Veterinary Medicine, Vienna, Austria
contact email	gokhlesh.kumar@vetmeduni.ac.at
lab head
Gokhlesh Kumar
contact affiliation	University of Veterinary Medicine, Vienna, Austria
contact email	Gokhlesh.Kumar@vetmeduni.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD008478

PRIDE project URI

• PRIDE
  1. PXD008478
     1. Label: PRIDE project
     2. Name: Shotgun proteomic profiling of spleen of rainbow trout Oncorhynchus mykiss