PXD008464

PXD008464 is an original dataset announced via ProteomeXchange.

Title	An integrated analysis of protein abundance, transcript level and tissue diversity to reveal developmental regulation of maize
Description	The differentiation and subsequent development of plant tissues or organs are tightly regulated at multiple levels, including the transcriptional, posttranscriptional, translational, and posttranslational levels. Transcriptomes define many of the tissue-specific gene expression patterns in maize, and some key genes and their regulatory networks have been established at the transcriptional level. In this study, the sequential window acquisition of all theoretical spectra-mass spectrometry technique was employed as a quantitative proteome assay of four representative maize tissues, and a set of high confidence proteins were identified. Integrated analysis of the proteome and transcriptome revealed that protein abundance was positively correlated with mRNA level with weak to moderate correlation coefficients, but the abundance of key proteins for function or architecture in a given tissue was closely tempo-spatially regulated at the transcription level. A subset of differentially expressed proteins, specifically tissue-specific proteins, were identified, e.g., reproductive structure and flower development-related proteins in tassel and ear, lipid and fatty acid biosynthetic process-related proteins in immature embryo, and inorganic substance and oxidation reduction responsive proteins in root, potentially revealing the physiology, morphology and function of each tissue. Furthermore, we found many new proteins in specific tissues that were highly correlated with their mRNA levels, in addition to known key factors. These proteome data provide new perspective for understanding many aspects of maize developmental biology.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:43.204.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jia Haitao
SpeciesList	scientific name: Zea mays (Maize); NCBI TaxID: 4577;
ModificationList	mono N-acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-15 01:30:36	ID requested
1	2018-01-02 02:44:18	announced
⏵ 2	2024-10-22 04:42:44	announced	2024-10-22: Updated project metadata.

10.1021/acs.jproteome.7b00586;

Jia H, Sun W, Li M, Zhang Z, Integrated Analysis of Protein Abundance, Transcript Level, and Tissue Diversity To Reveal Developmental Regulation of Maize. J Proteome Res, 17(2):822-833(2018) [pubmed]

curator keyword: Biological

submitter keyword: Tissue-specific proteins, Proteome, SWATH-MS,Differentially expressed proteins, Transcriptome, Zea mays L.

Zuxin Zhang
contact affiliation	National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China
contact email	zuxinzhang@mail.hzau.edu.cn
lab head
Jia Haitao
contact affiliation	National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R. China
contact email	jiahaitao.1986@163.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD008464

PRIDE project URI

• PRIDE
  1. PXD008464
     1. Label: PRIDE project
     2. Name: An integrated analysis of protein abundance, transcript level and tissue diversity to reveal developmental regulation of maize