PXD008459

PXD008459 is an original dataset announced via ProteomeXchange.

Title	Extensive identification and in-depth validation of importin 13 cargoes
Description	Importin 13 is a bidirectional transport receptor that mediates both nuclear import and export of proteins. To expand the range of human importin 13 cargoes, we performed importin 13 binding experiments in combination with quantitative stable isotope labeling with amino acids in cell culture (SILAC) and mass spectrometry. Apart from known importin 13 interaction partners, such as Ubc9 and eIF1A, more than 300 potential importin 13 cargoes were identified, several of which could be validated using cellular and biochemical assays.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:51.498.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Oliver Valerius
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-15 00:36:38	ID requested
1	2018-04-20 05:29:46	announced
⏵ 2	2024-10-22 04:42:59	announced	2024-10-22: Updated project metadata.

Baade I, Spillner C, Schmitt K, Valerius O, Kehlenbach RH, Extensive Identification and In-depth Validation of Importin 13 Cargoes. Mol Cell Proteomics, 17(7):1337-1353(2018) [pubmed]

10.1074/mcp.ra118.000623;

curator keyword: Biological

submitter keyword: importin 13, human, SILAC, nucleocytoplasmic transport, nuclear transport receptor

Ralph H. Kehlenbach
contact affiliation	University Medical Center Göttingen, Department of Molecular Biology, Germany
contact email	rkehlen@gwdg.de
lab head
Oliver Valerius
contact affiliation	Georg-August-Universität Göttingen
contact email	ovaleri@gwdg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008459

PRIDE project URI

• PRIDE
  1. PXD008459
     1. Label: PRIDE project
     2. Name: Extensive identification and in-depth validation of importin 13 cargoes