PXD008455

PXD008455 is an original dataset announced via ProteomeXchange.

Title	Proteomic Profile of Daphnia pulex using Data-Independent Acquisition Mass Spectrometry and Ion Mobility Separation
Description	In this study, various sample preparation and mass spectrometry acquisition methods were performed for the purpose of improving Daphnia pulex proteome exploration. We observed benefits for employing both in-gel and in-solution methods of trypsin digestion. Furthermore, data independent acquisition methods employing ion-mobility separation greatly increased peptide identification and more than doubled our proteome coverage.
HostingRepository	PRIDE
AnnounceDate	2018-07-11
AnnouncementXML	Submission_2018-07-11_03:48:18.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Gombar
SpeciesList	scientific name: Daphnia pulex (Water flea); NCBI TaxID: 6669;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	SYNAPT G2-Si

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-14 04:28:47	ID requested
⏵ 1	2018-07-11 03:48:19	announced

Steevensz A, Gombar R, Vergilino R, Cristescu ME, Vacratsis PO, Proteomic Profile of Daphnia pulex using Data-Independent Acquisition Mass Spectrometry and Ion Mobility Separation. Proteomics, 18(16):e1700460(2018) [pubmed]

curator keyword: Biological

submitter keyword: Daphnia pulex, proteomics, data independent acquisition, ion mobility, bioinformatics

Panayiotis, O., Vacratsis
contact affiliation	Chemistry and biochemistry, university of windsor, Canada
contact email	vacratsi@uwindsor.ca
lab head
Robert Gombar
contact affiliation	University of Windsor
contact email	gombarr@uwindsor.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD008455

PRIDE project URI

• PRIDE
  1. PXD008455
     1. Label: PRIDE project
     2. Name: Proteomic Profile of Daphnia pulex using Data-Independent Acquisition Mass Spectrometry and Ion Mobility Separation