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PXD008440

PXD008440 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe response to neoadjuvant chemoradiotherapy to 5-fluorouracil in locally advanced colorectal cancer patients: a predictive proteomic signature.
DescriptionBackground: Colorectal cancer is the third most common and the fourth most lethal cancer in the world. In the majority of cases, patients are diagnosed at an advanced stage or even metastatic, thus explaining the high mortality. The standard protocol for treating patients with locally advanced non-metastatic colorectal cancer (CRC) is neoadjuvant radio-chemotherapy (NRCT) with 5-fluorouracil (5-FU), but the resistance rate to this treatment remains high with approximately 30% of non-responders. The lack of evidence available in clinical practice to predict NRCT resistance to 5-FU and to guide clinical practice therefore encourages the search for biomarkers of this resistance. Methods: From twenty-three formalin-fixed paraffin-embedded (FFPE) biopsies performed before NRCT with 5-FU of locally advanced non-metastatic CRC patients, we extracted and analysed the tumor proteome of these patients. From clinical data, we were able to classify the twenty-two patients in our cohort into three treatment response groups: non-responders (NR), partial responders (PR) and total responders (TR), and to compare the proteomes of these different groups. Results: We have highlighted 384 differentially expressed proteins between NR and PR, 248 between NR and TR and 417 between PR and TR. Among these proteins, we have identified many differentially expressed proteins identified as having a role in cancer (IFIT1, FASTKD2, PIP4K2B, ARID1B, SLC25A33: overexpressed in TR; CALD1, CPA3, B3GALT5, CD177, RIPK1: overexpressed in NR). We have also identified that DPYD, the main degradation enzyme of 5-FU, was overexpressed in NR, as well as several ribosomal and mitochondrial proteins also overexpressed in NR. Conclusions: From these retrospective study, we implemented a protein extraction protocol from FFPE biopsy to highlight protein differences between different response groups to RCTN with 5-FU in patients with locally advanced non-metastatic CRC. These results will pave the way for a larger cohort for better sensitivity and specificity of the signature to guide decisions in the choice of treatment.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:42:25.814.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterFrancois-Michel Boisvert
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-12-13 08:22:58ID requested
12018-06-25 08:37:19announced
22024-10-22 04:42:34announced2024-10-22: Updated project metadata.
Publication List
Chauvin A, Wang CS, Geha S, Garde-Granger P, Mathieu AA, Lacasse V, Boisvert FM, The response to neoadjuvant chemoradiotherapy with 5-fluorouracil in locally advanced rectal cancer patients: a predictive proteomic signature. Clin Proteomics, 15():16(2018) [pubmed]
10.1186/s12014-018-9192-2;
Keyword List
curator keyword: Biomedical
submitter keyword: 5-fluorouracil, proteomics,colorectal cancer, resistance to neoadjuvant radio-chemotherapy, mass spectrometry, predictive biomarkers
Contact List
Francois-Michel Boisvert
contact affiliationDepartment of Anatomy and Cell Biology, Faculty of Medecine and Health Sciences, Université de Sherbrooke, Sherbrooke, Québec, Canada
contact emailfm.boisvert@usherbrooke.ca
lab head
Francois-Michel Boisvert
contact affiliationUniversité de Sherbrooke
contact emailfm.boisvert@usherbrooke.ca
dataset submitter
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