PXD008438

PXD008438 is an original dataset announced via ProteomeXchange.

Title	lysosome proteomics in Niemann-Pick Type C disease model cells (CHOwt and NPC1-null cells)
Description	Increasing evidence implicates lysosomal dysfunction in the pathogenesis of neurodegenerative diseases, including the rare inherited lysosomal storage disorders (LSDs) and the most common neurodegenerative diseases, such as Alzheimer’s and Parkinson’s disease (AD and PD). The goal of this work was to analyze whether there are changes in the lysosomal glycocalyx in a cellular model of a LSD Niemann-Pick type C disease (NPC). Using the ferrofluid nanoparticles we isolated lysosomal organelles from NPC1-null and CHOwt cells. Mass spectrometry identification of lysosomal proteins was performed in order to determine the enrichment efficiency for N-glycome profiling of the lysosomal glycocalyx in NPC disease cellular model.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:33:57.180.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anita Horvatic
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-13 05:27:51	ID requested
1	2018-03-21 02:02:18	announced
⏵ 2	2024-10-22 04:34:03	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra117.000129;

Kosicek M, Gudelj I, Horvatic A, Jovic T, Vuckovic F, Lauc G, Hecimovic S, N-glycome of the Lysosomal Glycocalyx is Altered in Niemann-Pick Type C Disease (NPC) Model Cells. Mol Cell Proteomics, 17(4):631-642(2018) [pubmed]

ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Glycoproteomics (B/D-HPP)

submitter keyword: magnetic nanoparticles lysosome enrichment, NPC1-null cells, CHOwt cells,lysosome, LC-MS/MS

Silva Katusic Hecimovic
contact affiliation	Laboratory for Neurodegenerative Disease Research Division of Molecular Medicine Rudjer Boskovic Institute Bijenicka 54, 10000 Zagreb - Croatia
contact email	silva.hecimovic@irb.hr
lab head
Anita Horvatic
contact affiliation	University of Zagreb, Faculty of Veterinary Medicine
contact email	horvatic.ani@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008438

PRIDE project URI

• PRIDE
  1. PXD008438
     1. Label: PRIDE project
     2. Name: lysosome proteomics in Niemann-Pick Type C disease model cells (CHOwt and NPC1-null cells)