⮝ Full datasets listing

PXD008438

PXD008438 is an original dataset announced via ProteomeXchange.

Dataset Summary
Titlelysosome proteomics in Niemann-Pick Type C disease model cells (CHOwt and NPC1-null cells)
DescriptionIncreasing evidence implicates lysosomal dysfunction in the pathogenesis of neurodegenerative diseases, including the rare inherited lysosomal storage disorders (LSDs) and the most common neurodegenerative diseases, such as Alzheimer’s and Parkinson’s disease (AD and PD). The goal of this work was to analyze whether there are changes in the lysosomal glycocalyx in a cellular model of a LSD Niemann-Pick type C disease (NPC). Using the ferrofluid nanoparticles we isolated lysosomal organelles from NPC1-null and CHOwt cells. Mass spectrometry identification of lysosomal proteins was performed in order to determine the enrichment efficiency for N-glycome profiling of the lysosomal glycocalyx in NPC disease cellular model.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:33:57.180.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterAnita Horvatic
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-12-13 05:27:51ID requested
12018-03-21 02:02:18announced
22024-10-22 04:34:03announced2024-10-22: Updated project metadata.
Publication List
10.1074/mcp.ra117.000129;
Kosicek M, Gudelj I, Horvatic A, Jovic T, Vuckovic F, Lauc G, Hecimovic S, N-glycome of the Lysosomal Glycocalyx is Altered in Niemann-Pick Type C Disease (NPC) Model Cells. Mol Cell Proteomics, 17(4):631-642(2018) [pubmed]
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Glycoproteomics (B/D-HPP)
submitter keyword: magnetic nanoparticles lysosome enrichment, NPC1-null cells, CHOwt cells,lysosome, LC-MS/MS
Contact List
Silva Katusic Hecimovic
contact affiliationLaboratory for Neurodegenerative Disease Research Division of Molecular Medicine Rudjer Boskovic Institute Bijenicka 54, 10000 Zagreb - Croatia
contact emailsilva.hecimovic@irb.hr
lab head
Anita Horvatic
contact affiliationUniversity of Zagreb, Faculty of Veterinary Medicine
contact emailhorvatic.ani@gmail.com
dataset submitter
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008438
PRIDE project URI
Repository Record List
[ + ]