PXD008438 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | lysosome proteomics in Niemann-Pick Type C disease model cells (CHOwt and NPC1-null cells) |
Description | Increasing evidence implicates lysosomal dysfunction in the pathogenesis of neurodegenerative diseases, including the rare inherited lysosomal storage disorders (LSDs) and the most common neurodegenerative diseases, such as Alzheimer’s and Parkinson’s disease (AD and PD). The goal of this work was to analyze whether there are changes in the lysosomal glycocalyx in a cellular model of a LSD Niemann-Pick type C disease (NPC). Using the ferrofluid nanoparticles we isolated lysosomal organelles from NPC1-null and CHOwt cells. Mass spectrometry identification of lysosomal proteins was performed in order to determine the enrichment efficiency for N-glycome profiling of the lysosomal glycocalyx in NPC disease cellular model. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:33:57.180.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Anita Horvatic |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-12-13 05:27:51 | ID requested | |
1 | 2018-03-21 02:02:18 | announced | |
⏵ 2 | 2024-10-22 04:34:03 | announced | 2024-10-22: Updated project metadata. |
Publication List
10.1074/mcp.ra117.000129; |
Kosicek M, Gudelj I, Horvatic A, Jovic T, Vuckovic F, Lauc G, Hecimovic S, N-glycome of the Lysosomal Glycocalyx is Altered in Niemann-Pick Type C Disease (NPC) Model Cells. Mol Cell Proteomics, 17(4):631-642(2018) [pubmed] |
Keyword List
ProteomeXchange project tag: Biology/Disease-Driven Human Proteome Project (B/D-HPP), Human Proteome Project, Glycoproteomics (B/D-HPP) |
submitter keyword: magnetic nanoparticles lysosome enrichment, NPC1-null cells, CHOwt cells,lysosome, LC-MS/MS |
Contact List
Silva Katusic Hecimovic |
contact affiliation | Laboratory for Neurodegenerative Disease Research Division of Molecular Medicine Rudjer Boskovic Institute Bijenicka 54, 10000 Zagreb - Croatia |
contact email | silva.hecimovic@irb.hr |
lab head | |
Anita Horvatic |
contact affiliation | University of Zagreb, Faculty of Veterinary Medicine |
contact email | horvatic.ani@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008438
- Label: PRIDE project
- Name: lysosome proteomics in Niemann-Pick Type C disease model cells (CHOwt and NPC1-null cells)