PXD008435

PXD008435 is an original dataset announced via ProteomeXchange.

Title	Proteomic identification of Galectin-11 and 14 ligands from Haemonchus contortus
Description	Haemonchus contortus is the most pathogenic nematode of small ruminants. Infection in sheep and goats results in anaemia that decreases animal productivity and can ultimately cause death. The involvement of ruminant-specific galectin-11 (LGALS-11) and galectin-14 (LGALS-14) has been postulated to play important roles in protective immune responses against parasitic infection; however, their ligands are unknown. In the current study, LGALS-11 and LGALS-14 ligands in H. contortus were identified from larval (L4) and adult parasitic stages extracts using immobilised LGALS-11 and LGALS-14 affinity column chromatography and mass spectrometry. Both LGALS-11 and LGALS-14 bound more putative protein targets in the adult stage of H. contortus (43 proteins) when compared to the larval stage (2 proteins). Of the 43 proteins identified in the adult stage, 34 and 35 proteins were bound by LGALS-11 and LGALS-14, respectively, with 26 proteins binding to both galectins. Interestingly, hematophagous stage-specific sperm-coating protein and zinc metalloprotease (M13), which are known vaccine candidates, were identified as putative ligands of both LGALS-11 and LGALS-14. The identification of glycoproteins of H. contortus by LGALS-11 and LGALS-14 provide new insights into host-parasite interactions and the potential for developing new interventions.
HostingRepository	PRIDE
AnnounceDate	2018-02-28
AnnouncementXML	Submission_2018-04-25_06:18:44.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008435
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pierre Faou
SpeciesList	scientific name: Haemonchus contortus (Barber pole worm); NCBI TaxID: 6289;
ModificationList	Oxidation; Carbamidomethyl
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-13 03:17:11	ID requested
1	2018-02-28 06:03:33	announced
⏵ 2	2018-04-25 06:18:46	announced	Updated publication reference for PubMed record(s): 29576976.

Sakthivel D, Swan J, Preston S, Shakif-Azam MD, Faou P, Jiao Y, Downs R, Rajapaksha H, Gasser R, Piedrafita D, Beddoe T, . PeerJ, 6():e4510(2018) [pubmed]

curator keyword: Biological

submitter keyword: Proteomics

Pierre Faou
contact affiliation	La Trobe university
contact email	P.faou@latrobe.edu.au
lab head
Pierre Faou
contact affiliation	La Trobe University
contact email	P.faou@latrobe.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008435
     1. Label: PRIDE project
     2. Name: Proteomic identification of Galectin-11 and 14 ligands from Haemonchus contortus