PXD008404

PXD008404 is an original dataset announced via ProteomeXchange.

Title	Comparison of Nuclear Matrix and Mitotic Chromosome Scaffold proteins in Drosophila S2 cells – Transmission of hallmarks of nuclear organization through mitosis
Description	During open mitosis chromatin condenses to form chromosomes and decondenses post-mitotically to re-occupy their designated nuclear territory in a precisely lineage specific manner. This necessitates that features of nuclear architecture persist through mitosis. Here we present a proteomic study, which shows that the features of nuclear architecture in interphase nucleus are retained on the mitotic chromosome. Proteomic comparison was made between nuclease and high salt resistant fraction of interphase nucleus known as nuclear matrix (NuMat) and an identical biochemical fraction in the mitotic chromosome known as mitotic chromosome scaffold (MiCS). Our study elucidates that a large graction of the NuMat proteins are retained in the MiCS and possibly play an important role in maintenance of cell lineage specific features of nuclear architecture during cell division and thereby serve as components of cellular memory.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:18.600.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	RAHUL SUREKA
SpeciesList	scientific name: Drosophila melanogaster (Fruit fly); NCBI TaxID: 7227;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-11 01:50:13	ID requested
1	2018-07-17 08:35:37	announced
⏵ 2	2024-10-22 04:42:26	announced	2024-10-22: Updated project metadata.

10.1074/mcp.ra118.000591;

Sureka R, Wadhwa R, Thakur SS, Pathak RU, Mishra RK, S2 Cells-Transmission of Hallmarks of Nuclear Organization Through Mitosis. Mol Cell Proteomics, 17(10):1965-1978(2018) [pubmed]

curator keyword: Biological

submitter keyword: Nuclear Matrix, Drosophila melanogastor, S2 cells, Nuclear architecture, Mitotic chromosome scaffold, Mitosis

Rakesh Kumar Mishra
contact affiliation	CSIR-Centre for Cellular and Molecular Biology Hyderabad India
contact email	mishra@ccmb.res.in
lab head
RAHUL SUREKA
contact affiliation	CSIR-CENTRE FOR CELLULAR AND MOLECULAR BIOLOGY
contact email	rahul@ccmb.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD008404

PRIDE project URI

• PRIDE
  1. PXD008404
     1. Label: PRIDE project
     2. Name: Comparison of Nuclear Matrix and Mitotic Chromosome Scaffold proteins in Drosophila S2 cells – Transmission of hallmarks of nuclear organization through mitosis