PXD008399

PXD008399 is an original dataset announced via ProteomeXchange.

Title	Direct identification of protein S-palmitoylation sites
Description	S-fatty-acylation is the covalent attachment of long chain fatty acids, predominately palmitate (C16:0, S-palmitoylation), to cysteine (Cys) residues via a thioester linkage on proteins. This post-translational and reversible lipid modification regulates protein function and localization in eukaryotes and is important in mammalian physiology and human disease. While chemical labeling methods have improved the detection and enrichment of S-fatty-acylated proteins, mapping sites of modification and characterization of endogenously attached fatty acids is still challenging. Here, we describe the integration and optimization of fatty acid chemical reporter labeling with hydroxylamine-mediated enrichment of S-fatty-acylated proteins and direct tagging of modified Cys residues to selectively map lipid modification sites. This afforded improved enrichment and direct identification of many protein S-fatty-acylation sites compared to previously described methods.
HostingRepository	PRIDE
AnnounceDate	2018-03-28
AnnouncementXML	Submission_2018-03-28_00:01:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emmanuelle Thinon
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	palmitoylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-11 01:24:59	ID requested
⏵ 1	2018-03-28 00:01:53	announced

Thinon E, Fernandez JP, Molina H, Hang HC, Selective Enrichment and Direct Analysis of Protein S-Palmitoylation Sites. J Proteome Res, 17(5):1907-1922(2018) [pubmed]

curator keyword: Biological

submitter keyword: S-fatty-acylation, S-palmitoylation, site identification

Howard C Hang
contact affiliation	Laboratory of Chemical Biology and Microbial Pathogenesis, The Rockefeller University, New York, NY 10065, USA
contact email	hhang@rockefeller.edu
lab head
Emmanuelle Thinon
contact affiliation	The Francis Crick Institute
contact email	emmanuellethinon@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008399

PRIDE project URI

• PRIDE
  1. PXD008399
     1. Label: PRIDE project
     2. Name: Direct identification of protein S-palmitoylation sites