PXD008396

PXD008396 is an original dataset announced via ProteomeXchange.

Title	A comprehensive Proteomic Investigation of Ebf1 Heterozygosity in pro-B lymphocytes utilizing Data Independent Acquisition
Description	Early B cell factor 1 (EBF1) is one of the key transcription factors required for orchestrating B-cell lineage development. Although studies have shown that Ebf1 haploinsufficiency is involved in the development of leukemia, no study has been conducted that characterizes the global effect of Ebf1 heterozygosity on the proteome of pro-B lymphocytes. Here, we employ both DIA (Data Independent Acquisition) and shotgun DDA (Data Dependent Acquisition) workflows for profiling proteins that are differently expressed between Ebf1+/+ and Ebf1+/- cells. Both DDA and DIA were able to reveal the downregulation of the EBF1 transcription factor in Ebf1+/- pro-B lymphocytes. Further examination of differentially expressed proteins by DIA revealed that, similar to EBF1, the expression of other B-cell lineage regulators, such as TCF3 and Pax5, is also down-regulated in Ebf1 heterozygous cells. Functional DIA analysis of differentially expressed proteins showed that EBF1 heterozygosity resulted in the deregulation of at least 8 transcription factors involved in lymphopoiesis, and to the deregulation of key proteins playing crucial roles in survival, development and differentiation of pro-B lymphocytes.
HostingRepository	PRIDE
AnnounceDate	2017-12-20
AnnouncementXML	Submission_2017-12-20_06:59:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yaarub Musa
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-11 00:24:54	ID requested
⏵ 1	2017-12-20 06:59:17	announced

Musa YR, Boller S, Puchalska M, Grosschedl R, Mittler G, Comprehensive Proteomic Investigation of Ebf1 Heterozygosity in Pro-B Lymphocytes Utilizing Data Independent Acquisition. J Proteome Res, 17(1):76-85(2018) [pubmed]

curator keyword: Biological

submitter keyword: DDA, DIA, PRM, Ebf1, Pro-B lymphocyte, transcription factor

Gerhard Mittler
contact affiliation	Proteomics Facility Max Planck Insitutte for Immunobiology and Epigenetics
contact email	mittler@ie-freiburg.mpg.de
lab head
Yaarub Musa
contact affiliation	Max Planck Institute for Immunobiology and Epigenetics
contact email	musa@ie-freiburg.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008396
     1. Label: PRIDE project
     2. Name: A comprehensive Proteomic Investigation of Ebf1 Heterozygosity in pro-B lymphocytes utilizing Data Independent Acquisition