PXD008380

PXD008380 is an original dataset announced via ProteomeXchange.

Title	Labelling and identifying cell-specific proteomes in the mouse brain
Description	Defining the in vivo proteomes of specific cell-types in the central nervous system of mammals, without ex vivo manipulation, is an important and outstanding challange. Here we demonstrate that stochastic orthogonal recoding of translation (SORT) can be used to tag the proteomes of specific cell types in brains of live mice. We tag neuronal proteomes from the striatum and perform enrichment before identifing neuronal proteins by LC-MSMS.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:41:20.451.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Toke Hansen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-06 07:40:40	ID requested
1	2017-12-13 04:21:48	announced
2	2017-12-19 02:08:02	announced	Updated publication reference for PubMed record(s): 29251727.
⏵ 3	2024-10-22 04:41:21	announced	2024-10-22: Updated project metadata.

10.1038/nbt.4056;

Krogager TP, Ernst RJ, Elliott TS, Calo L, Ber, á, nek V, Ciabatti E, Spillantini MG, Tripodi M, Hastings MH, Chin JW, Labeling and identifying cell-specific proteomes in the mouse brain. Nat Biotechnol, 36(2):156-159(2018) [pubmed]

curator keyword: Technical, Biological

submitter keyword: In-vivo , Neuron, Mouse, Unnatrual amino acid, Enrichment, Labelling

Jason Chin
contact affiliation	MRC-Laboratory of Molecular Biology,Cambridge,UK.
contact email	chin@mrc-lmb.cam.ac.uk
lab head
Toke Hansen
contact affiliation	MRC-LMB
contact email	thansen@mrc-lmb.cam.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2017/12/PXD008380

PRIDE project URI

• PRIDE
  1. PXD008380
     1. Label: PRIDE project
     2. Name: Labelling and identifying cell-specific proteomes in the mouse brain