PXD008378 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Proteomic characterization of CD34+ CD123+ stem cells from patients with acute myeloid leukemia |
Description | The comparative characterization of hematopoietic stem cells from healthy stem cell donors and patients with acute myeloid leukemia on a proteome level has the potential to reveal differentially regulated proteins which might be candidates for specific immunotherapy target molecules. Exemplarily, we analyzed the proteome of the cytosolic and the membrane fraction of CD34 and CD123 co-expressing FACS-sorted leukemic progenitors from five patients with acute myeloid leukemia employing mass spectrometry. As a reference, CD34+CD123+ normal hematopoietic progenitor cells from five healthy stem cell donors were analyzed. In this TMT 10-plex labeling based approach 2068 proteins were identified with 256 proteins differentially regulated in one or both cellular compartments. This study demonstrates the feasibility of a mass spectrometry based proteomic approach to detect differentially expressed proteins in two compartment fractions of leukemic stem cells as compared to their healthy stem cell counterparts. |
HostingRepository | PRIDE |
AnnounceDate | 2018-02-19 |
AnnouncementXML | Submission_2018-02-19_02:46:11.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Johannes R. Schmidt |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-12-06 05:59:44 | ID requested | |
⏵ 1 | 2018-02-19 02:46:12 | announced | |
Publication List
Schmidt JR, R, ü, cker-Braun E, Heidrich K, von Bonin M, St, ö, lzel F, Thiede C, Middeke JM, Ehninger G, Bornh, ä, user M, Schetelig J, Schubert K, von Bergen M, Heidenreich F, Progenitor Cells for the Identification of Potential Targets for Immunotherapy in Acute Myeloid Leukemia. Proteomes, 6(1):(2018) [pubmed] |
Keyword List
curator keyword: Biomedical |
submitter keyword: acute myeloid leukemia, AML, LC-MS, proteomics |
Contact List
Martin von Bergen |
contact affiliation | Department for Molecular Systems Biology, Helmholtz Centre for Environmental Research - UFZ, Leipzig, Germany |
contact email | martin.vonbergen@ufz.de |
lab head | |
Johannes R. Schmidt |
contact affiliation | Helmholtz Centre for Environmental Research - UFZ |
contact email | johannes.schmidt@ufz.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008378
- Label: PRIDE project
- Name: Proteomic characterization of CD34+ CD123+ stem cells from patients with acute myeloid leukemia