PXD008376

PXD008376 is an original dataset announced via ProteomeXchange.

Title	Integrated transcriptomic and proteomic analyses revealed the participation of endogenous protease inhibitors in the regulation of protease gene expression in Helicoverpa armigera
Description	Insects adapt to plant protease inhibitors (PIs) present in their diet by differentially regulating multiple digestive proteases. However, mechanisms regulating protease gene expression in insects are largely enigmatic. Ingestion of Capsicum annuum protease inhibitor-7 (CanPI-7) arrests growth and development of Helicoverpa armigera (Lepidoptera: noctuidae). Using de novo RNA sequencing and proteomic analysis, we examined the response of H. armigera larvae fed on recombinant C. annuum PI (CanPI) at different time intervals. Here, we present evidence supporting a dynamic transition in H. armigera protease expression upon CanPI feeding with general down-regulation of protease genes at early time points (0.5 to 6 h) and significant up-regulation of specific trypsin, chymotrypsin and aminopeptidase genes at later time points (12 to 48 h).
HostingRepository	PRIDE
AnnounceDate	2018-04-25
AnnouncementXML	Submission_2018-04-25_00:25:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ashok Giri
SpeciesList	scientific name: Helicoverpa armigera (Cotton bollworm); NCBI TaxID: 29058;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-06 04:37:55	ID requested
⏵ 1	2018-04-25 00:25:39	announced

Lomate PR, Dewangan V, Mahajan NS, Kumar Y, Kulkarni A, Wang L, Saxena S, Gupta VS, Giri AP, . Mol Cell Proteomics, 17(7):1324-1336(2018) [pubmed]

curator keyword: Biological

submitter keyword: Protease regulation, Helicoverpa armigera, plant protease inhibitor, transcriptome, insect adaptation, SWATH, TripleTOF 5600

Ashok P. Giri
contact affiliation	Principal Scientist and Chair, Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune, INDIA
contact email	ap.giri@ncl.res.in
lab head
Ashok Giri
contact affiliation	Principal Scientist and Chair, Biochemical Sciences Division, CSIR-National Chemical Laboratory, Pune, INDIA
contact email	ap.giri@ncl.res.in
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008376

PRIDE project URI

• PRIDE
  1. PXD008376
     1. Label: PRIDE project
     2. Name: Integrated transcriptomic and proteomic analyses revealed the participation of endogenous protease inhibitors in the regulation of protease gene expression in Helicoverpa armigera