PXD008374

PXD008374 is an original dataset announced via ProteomeXchange.

Title	Comparative phosphoproteomic and acetylated proteomic analysis of intestine regeneration in the sea cucumber Apostichopus japonicus
Description	Sea cucumbers have a striking capacity to regeneratemost of their viscera after evisceration, which has drawn theinterest ofmany researchers. In this study, we perform quantitative phosphoproteomics proteomic to analyze the proteinphosphorylation and acetylation changes between regenerative intestine at 3 days post evisceration (dpe) and normal intestine (intact intestine in normal situation) in A.japonicus. Altogether, 2,584 phosphorylation sites in 1,531 proteins were identified, among which 1862 phosphorylation site in 1169 proteins were quantified. When quantitative ratio of phosphorylation sites over 1.5 was considered up-regulation while quantitative ratio of phosphorylation sites below 0.67 (1/1.5) was considered as down-regulation. The amount of the up-regulatedphosphorylation quantified sites and proteins were 127 and 113; the amount of the down-regulatedphosphorylation quantified sites and proteins were 38 and 34. Totally, 886acetylation sites in 555 proteins were identified, among which 712acetylation site in 470 proteins were quantified (Table 1). When quantitative ratio of acetylation sites over 1.20 was considered up-regulation while quantitative ratio of acetylation sites below 0.83 (1/1.2) was considered as down-regulation. The amount of the up-regulatedacetylation quantified sites and proteins were 130 and 101; the amount of the down-regulatedacetylation quantified sites and proteins were 211 and 150.Our results suggest that intestine regeneration constitutes a complex lifeactivity regulated by the cooperation of various biological processes, including cytoskeletal changes, protein synthesis, signal recognition and transduction,energy production and conversion, and substance transport andmetabolism.
HostingRepository	PRIDE
AnnounceDate	2018-10-22
AnnouncementXML	Submission_2018-10-22_09:34:40.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rachel Green
SpeciesList	scientific name: Stichopus japonicus (Sea cucumber); NCBI TaxID: 307972;
ModificationList	phosphorylated residue; acetylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-06 03:36:53	ID requested
⏵ 1	2018-10-22 09:34:41	announced

Sun L, Lin C, Li X, Xing L, Huo D, Sun J, Zhang L, Yang H, Comparative Phospho- and Acetyl Proteomics Analysis of Posttranslational Modifications Regulating Intestine Regeneration in Sea Cucumbers. Front Physiol, 9():836(2018) [pubmed]

curator keyword: Biological

submitter keyword: Sea cucumber, Regeneration, Phosphoproteomic, Acetylated proteomic, Protein modified regulation

Lina Sun
contact affiliation	Institute of Oceanology, Chinese Academy of Sciences
contact email	sunlina@qdio.ac.cn
lab head
Rachel Green
contact affiliation	PTM Biolabs Inc.
contact email	xuan_mao@ptm-biolab.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008374
     1. Label: PRIDE project
     2. Name: Comparative phosphoproteomic and acetylated proteomic analysis of intestine regeneration in the sea cucumber Apostichopus japonicus