PXD008370

PXD008370 is an original dataset announced via ProteomeXchange.

Title	Sequential enrichment of phosphorylation on reversibly oxidized proteoforms – part 1
Description	Post-translational modifications (PTMs) are necessary for the regulation of critical processes including metabolism, signaling, and overall homeostasis. While proteins PTMs have been largely investigated independently in bottom-up proteomics methodologies, examination into how different PTMs interact, or crosstalk, will reveal a more complete understanding of the reciprocity of signaling cascades across numerous pathways. Combinatorial reversible thiol oxidation and phosphorylation in eukaryotes is largely recognized, but rigorous approaches for experimental verification are underdeveloped and must be advanced to begin meaningful definition of crosstalk in targeted pathway and systems biology research. Herein, we applied protein-level enrichment of reversibly oxidized proteoforms in Chlamydomonas reinhardtii with subsequent phosphopeptide analysis to determine the extent of phosphorylation in the redox thiol proteome.
HostingRepository	PRIDE
AnnounceDate	2018-04-23
AnnouncementXML	Submission_2018-04-23_05:13:22.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008370
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Evan McConnell
SpeciesList	scientific name: Chlamydomonas reinhardtii; NCBI TaxID: 3055;
ModificationList	Phospho; Deamidated; Oxidation; Acetyl; Carbamidomethyl
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-05 06:09:35	ID requested
⏵ 1	2018-04-23 05:13:23	announced

McConnell EW, Werth EG, Hicks LM, The phosphorylated redox proteome of Chlamydomonas reinhardtii: Revealing novel means for regulation of protein structure and function. Redox Biol, 17():35-46(2018) [pubmed]

curator keyword: Biological

submitter keyword: Chlamydomonas reinhardtii, cross-talk, phosphorylation, redox proteomics, redox regulation

Leslie M. Hicks
contact affiliation	Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 USA
contact email	lmhicks@email.unc.edu
lab head
Evan McConnell
contact affiliation	Graduate Research Assistant
contact email	evanmcc@live.unc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008370
     1. Label: PRIDE project
     2. Name: Sequential enrichment of phosphorylation on reversibly oxidized proteoforms – part 1