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PXD008354

PXD008354 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleThe Proteomic Landscape in the Vitreous of Patients With Age-Related and Diabetic Retinal Disease
DescriptionIn contrast to neovascular age-related macular degeneration (nAMD), no treatment option exists for dry AMD. The identification of specific biomarkers is required to facilitate diagnosis and therapy of dry AMD. We thus measured the proteome of 34 vitreous humor samples (dry AMD: n = 6, nAMD: n = 10, PDR: n = 9, ERM: n = 9). Samples were immunodepleted for high abundant blood proteins and analyzed by LC-MS/MS with a shotgun approach. Data from dry AMD, nAMD and PDR were compared to the ERM patient group, which was used as reference group. A bioinformatic pipeline was used for label-free relative quantification of proteins and to perform cluster analysis, gene ontology classification and gene set enrichment analysis. A selection of differentially regulated proteins was validated by ELISA. A total of 677 proteins were identified and relatively quantified in the four patient groups. Different clusters of regulated proteins for each patient group were identified and showed characteristic enrichment of specific pathways, like ‘oxidative stress’ for dry AMD, ‘focal adhesion’ for nAMD and ‘complement and coagulation cascade’ for PDR patients. We’ve identified CHLE to be specifically upregulated in dry AMD and RNAS1 together with CPVL to be upregulated in both forms of AMD. The description of pathways specific for the different patient groups and the identification of differentially regulated proteins provide a first step towards the definition of biomarkers for dry AMD. To validate these data and to unravel the mechanistic connection of identified proteins to dry AMD, larger patient cohorts need to be investigated.
HostingRepositoryPRIDE
AnnounceDate2023-11-14
AnnouncementXMLSubmission_2023-11-14_08:49:36.686.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Schori
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListmonohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
InstrumentOrbitrap Fusion
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-12-04 00:48:12ID requested
12018-07-23 08:21:28announced
22018-07-23 23:58:33announcedUpdated project metadata.
32018-07-24 00:14:03announcedUpdated project metadata.
42023-11-14 08:49:37announced2023-11-14: Updated project metadata.
Publication List
Schori C, Trachsel C, Grossmann J, Zygoula I, Barthelmes D, Grimm C, The Proteomic Landscape in the Vitreous of Patients With Age-Related and Diabetic Retinal Disease. Invest Ophthalmol Vis Sci, 59(4):AMD31-AMD40(2018) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: PDR, proliferative diabetic retinopathy, dry AMD, age-related macular degeneration, neovascular AMD, epiretinal membrane, vitreous proteome,human, biomarker, vitreous humor, nAMD, ERM
Contact List
Christian Grimm
contact affiliationLab for Retinal Cell Biology, Department of Ophthalmology, University of Zürich, Wagistrasse 14, Schlieren, CH 8952, Switzerland
contact emailcgrimm@opht.uzh.ch
lab head
Christian Schori
contact affiliationUniversity of Zurich
contact emailchristian.schori@usz.ch
dataset submitter
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