PXD008351

PXD008351 is an original dataset announced via ProteomeXchange.

Title	Human AHH1 cells heavy ion irradiation LC-MSMS
Description	Heavy ion radiotherapy has unfolded a great promise for cancer therapy. Understanding the cellular response mechanism to heavy ion radiation is required for exploring the measures of overcoming the devastating side effects. Here, we performed a quantitative proteomics to investigate the mechanism of carbon heavy ion irradiation on human AHH-1 lymphoblastoid cells. Totally we identified 4602 proteins and quantified 4569 proteins among which with high coverage on mitochondria. After stringent filtering, we found 290 proteins were significantly up-regulated and 16 proteins were down-regulated. Functional analysis revealed that these up-regulated proteins specifically enriched in the process of DNA damage repair, mitochondrial ribosome and especially mitochondrial respiratory chain, which occupied approximately fifty percent of the accumulated proteins. Bioinformatics analysis and functional study demonstrated that these up- regulated mitochondrial respiratory chain proteins enhanced ATP production and simultaneously ROS releasing. More importantly, the increased ROS led to organelle secondary injury and the lagged DNA double strand breaks. Consistently, the expression of antioxidant enzymes was up-regulated for free radical scavenging. The mechanism of lagged secondary injury originated from disturbing of mitochondrial respiratory chain, the “target” outside nuclear, explored here may provide a novel target for cell self-repairing against heavy ion radiation induced cellular damage.
HostingRepository	PRIDE
AnnounceDate	2018-11-07
AnnouncementXML	Submission_2018-11-07_01:12:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Peng-Cheng Fan
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-01 08:49:56	ID requested
⏵ 1	2018-11-07 01:12:21	announced

Fan PC, Zhang Y, Wang Y, Wei W, Zhou YX, Xie Y, Wang X, Qi YZ, Chang L, Jia ZP, Zhou Z, Guan H, Zhang H, Xu P, Zhou PK, Quantitative proteomics reveals mitochondrial respiratory chain as a dominant target for carbon ion radiation: Delayed reactive oxygen species generation caused DNA damage. Free Radic Biol Med, 130():436-445(2019) [pubmed]

ProteomeXchange project tag: Human Proteome Project

curator keyword: Biological

submitter keyword: Human, AHH1 cells, heavy ion radiation, LC-MSMS

Ping Xu
contact affiliation	State Key Laboratory of Proteomics, National Center for Protein Sciences Beijing, Beijing Proteome Research Center
contact email	xuping@mail.ncpsb.org
lab head
Peng-Cheng Fan
contact affiliation	State Key Laboratory of Proteomics, National Center for Protein Sciences Beijing, Beijing Proteome Research Center
contact email	fpch2004@hotmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/11/PXD008351

PRIDE project URI

• PRIDE
  1. PXD008351
     1. Label: PRIDE project
     2. Name: Human AHH1 cells heavy ion irradiation LC-MSMS