PXD008346

PXD008346 is an original dataset announced via ProteomeXchange.

Title	Genomes of trombidid mites reveal novel predicted allergens and laterally-transferred genes associated with secondary metabolism
Description	Background Trombidid mites have a unique lifecycle in which only the larval stage is ectoparasitic. In the superfamily Trombiculoidea (“chiggers”), the larvae feed preferentially on vertebrates, including humans. Species in the genus Leptotrombidium are vectors of a potentially fatal bacterial infection, scrub typhus, which affects 1 million people annually. Moreover, chiggers can cause pruritic dermatitis (trombiculiasis) in humans and domesticated animals. In the Trombidioidea (velvet mites), the larvae feed on other arthropods and are potential biological control agents for agricultural pests. Here, we present the first trombidid mites genomes, obtained both for a chigger, Leptotrombidium deliense, and for a velvet mite, Dinothrombium tinctorium. Results Sequencing was performed on the Illumina MiSeq platform. A 180 Mb draft assembly for D. tinctorium was generated from two paired-end and one mate-pair library using a single adult specimen. For L. deliense, a lower-coverage draft assembly (117 Mb) was obtained using pooled, engorged larvae with a single paired-end library. Remarkably, both genomes exhibited evidence of ancient lateral gene transfer from soil-derived bacteria or fungi. The transferred genes confer functions that are rare in animals, including terpene and carotenoid synthesis. Thirty-seven allergenic protein families were predicted in the L. deliense genome, of which nine were unique. Preliminary proteomic analyses identified several of these putative allergens in larvae. Conclusions Trombidid mite genomes appear to be more dynamic than those of other acariform mites. A priority for future research is to determine the biological function of terpene synthesis in this taxon and its potential for exploitation in disease control. Project was jointly supervised by Stuart Armstrong and Ben Makepeace.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:25.674.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008346
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Stuart Armstrong
SpeciesList	scientific name: Dinothrombium; NCBI TaxID: 58781; scientific name: Leptotrombidium deliense; NCBI TaxID: 299467;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-01 07:57:13	ID requested
1	2018-09-18 07:27:41	announced
2	2018-09-19 04:04:45	announced	Updated project metadata.
3	2019-02-26 08:16:28	announced	Updated project metadata.
⏵ 4	2024-10-22 04:42:27	announced	2024-10-22: Updated project metadata.

10.6019/PXD008346;

10.1093/gigascience/giy127;

Dong X, Chaisiri K, Xia D, Armstrong SD, Fang Y, Donnelly MJ, Kadowaki T, McGarry JW, Darby AC, Makepeace BL, Genomes of trombidid mites reveal novel predicted allergens and laterally transferred genes associated with secondary metabolism. Gigascience, 7(12):(2018) [pubmed]

curator keyword: Biological

submitter keyword: Trombidid mites,Genome,LC-MSMS,scrub typhus

Julian Hiscox
contact affiliation	Department of Infection Biology, Institute of Infection and Global Health, University of Liverpool, UK
contact email	Julian.Hiscox@liverpool.ac.uk
lab head
Stuart Armstrong
contact affiliation	Infection Biology
contact email	sarmstro@liv.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008346
     1. Label: PRIDE project
     2. Name: Genomes of trombidid mites reveal novel predicted allergens and laterally-transferred genes associated with secondary metabolism