PXD008343

PXD008343 is an original dataset announced via ProteomeXchange.

Title	CRISPR/Cas9-mediated knockout and endogenous complex analysis of Cluap1/ IFT38
Description	CRISPR/Cas9-mediated gene-editing allows manipulation of a gene of interest in its own chromosomal context. When applied to the analysis of protein interaction, and in contrast to an exogenous expression of a protein, these can be studied maintaining physiological stochiometries, topology and context. We have used CRISPR/Cas9-mediated recombination to Cluap1/ IFT38, a component of the intraflagellar transport complex B (IFT-B). Cluap1 has been implicated in human development as well as in cancer progression. Cluap1 loss of function results in early developmental defects with neural tube closure, sonic hedgehog signaling and left-right defects. Herein, we generated an endogenously tagged Cluap1 for protein complex analysis which was then correlated to the corresponding interactome determined by ectopic expression.
HostingRepository	PRIDE
AnnounceDate	2018-04-06
AnnouncementXML	Submission_2018-04-10_01:11:03.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tina Beyer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Orbitrap Fusion; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-12-01 00:29:20	ID requested
1	2018-04-04 06:07:58	announced
2	2018-04-06 00:38:59	announced	Updated project metadata.
⏵ 3	2018-04-10 01:11:04	announced	Updated project metadata.

Beyer T, Bolz S, Junger K, Horn N, Moniruzzaman M, Wissinger Y, Ueffing M, Boldt K, CRISPR/Cas9-mediated Genomic Editing of Cluap1/IFT38 Reveals a New Role in Actin Arrangement. Mol Cell Proteomics, 17(7):1285-1294(2018) [pubmed]

curator keyword: Technical, Biological

submitter keyword: CRISPR/Cas9, cilia, IFT, proteomics

Marius Ueffing
contact affiliation	Institute for Ophthalmic Research, Centre for Ophthalmology, University of Tübingen, Germany
contact email	Marius.Ueffing@uni-tuebingen.de
lab head
Tina Beyer
contact affiliation	University Clinics Tuebingen
contact email	tina.beyer@uni-tuebingen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008343

PRIDE project URI

• PRIDE
  1. PXD008343
     1. Label: PRIDE project
     2. Name: CRISPR/Cas9-mediated knockout and endogenous complex analysis of Cluap1/ IFT38