PXD008334

PXD008334 is an original dataset announced via ProteomeXchange.

Title	Exportin Crm1 is repurposed as a docking protein to generate microtubule organizing centers at the nuclear pore
Description	Non-centrosomal microtubule organizing centers (MTOCs) are important for microtubule organization in many cell types. In fission yeast Schizosaccharomyces pombe, the protein Mto1, together with partner protein Mto2 (Mto1/2 complex), recruits the g-tubulin complex to multiple non-centrosomal MTOCs, including the nuclear envelope (NE). Here, we develop a comparative-interactome mass spectrometry approach to determine how Mto1 localizes to the NE. Surprisingly, we find that Mto1, a constitutively cytoplasmic protein, docks at nuclear pore complexes (NPCs), via interaction with exportin Crm1 and cytoplasmic FG-nucleoporin Nup146. Although Mto1 is not a nuclear export cargo, it binds Crm1 via a nuclear export signal-like sequence, and docking requires both Ran in the GTP-bound state and Nup146 FG repeats. In addition to determining the mechanism of MTOC formation at the NE, our results reveal a novel role for Crm1 and the nuclear export machinery in the stable docking of a cytoplasmic protein complex at NPCs.
HostingRepository	PRIDE
AnnounceDate	2018-05-22
AnnouncementXML	Submission_2019-02-26_08:17:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kenneth Sawin
SpeciesList	scientific name: Schizosaccharomyces pombe; NCBI TaxID: 4896;
ModificationList	No PTMs are included in the dataset
Instrument	Orbitrap Fusion Lumos; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-30 01:18:11	ID requested
1	2018-05-22 06:37:13	announced
⏵ 2	2019-02-26 08:17:06	announced	Updated publication reference for PubMed record(s): 29809148.

Bao XX, Spanos C, Kojidani T, Lynch EM, Rappsilber J, Hiraoka Y, Haraguchi T, Sawin KE, Exportin Crm1 is repurposed as a docking protein to generate microtubule organizing centers at the nuclear pore. Elife, 7():(2018) [pubmed]

curator keyword: Biological

submitter keyword: Schizosaccharomyces pombe, microtubules, MTOCs, nuclear envelope, Mto1, Mto2, Crm1, Nup146

Kenneth Sawin
contact affiliation	WTCCB, University of Edinburgh
contact email	ken.sawin@ed.ac.uk
lab head
Kenneth Sawin
contact affiliation	University of Edinburgh
contact email	ken.sawin@ed.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/05/PXD008334

PRIDE project URI

• PRIDE
  1. PXD008334
     1. Label: PRIDE project
     2. Name: Exportin Crm1 is repurposed as a docking protein to generate microtubule organizing centers at the nuclear pore