PXD008331
PXD008331 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Glioma initiating/stem cells, differentiation, iTRAQ analysis by TT5600 |
| Description | Glioma initiating/stem cells (GIC/GSC) are considered responsible for the therapeutic resistance and recurrence of malignant glioma. To clarify the molecular mechanism of GIC/GSC maintenance/differentiation, we established GIC/GSC clones, having the potential to differentiate into malignant gliomas, from glioblastoma patient’s tissues, and subjected to DNA microarray/iTRAQ based integrated proteomics. 21,857 mRNAs and 8,471 proteins were identified and integrated into a gene/protein expression analysis chart (iPEACH). The data integration and extraction of global proteins and mRNAs revealed that, during the GIC/GSC differentiation, cell adhesion molecules including integrin aV/ECMs and RAS-MAPK/PI3K signalings were significantly up-regulated, meanwhile, SOX2, CD133, and specific proteoglycans/synthetic-enzymes/metabolic pathways were obviously down-regulated. Among them, we focused proteoglycans and their synthetic-enzymes. GIC/GSC differentiation was significantly associated with the decrease of CSPG (CS-modified form) and dramatically induced by the CS-degradation enzyme which also induces the up-regulation of ERK-AKT signaling and GFAP without any other agents. Importantly, these differentiation processes were also associated with the interaction of CSPG (CS-unmodified form) and integrin-aV, and suppressed by integrin-inhibitors/CS administrations significantly. Combination treatments of a cancer-drug Temozolomide and these GIC/GSC-differentiation inhibitors suppressed glioma progression, increased the chemosensitivities, and led the longer survival of mouse xenograft-models. Functional integrated proteomics for the first time demonstrates that the GIC/GSC induces the specific proteoglycans to regulate GIC/GSC stemness/differentiation via the integlin signalings which may be a clinical target against malignant gliomas. |
| HostingRepository | jPOST |
| AnnounceDate | 2018-11-30 |
| AnnouncementXML | Submission_2022-09-18_03:16:54.812.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Non peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | Akiko Nambu |
| SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
| ModificationList | iTRAQ8plex reporter+balance reagent N-acylated residue; iTRAQ8plex reporter+balance reagent acylated N-terminal; L-methionine sulfoxide; L-cysteine methyl disulfide |
| Instrument | TripleTOF 5600 |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2017-11-30 00:56:40 | ID requested | |
| 1 | 2018-11-29 07:00:05 | announced | |
| 2 | 2021-02-04 17:12:19 | announced | 2021-02-04: Updated FTP location. |
| ⏵ 3 | 2022-09-18 03:16:55 | announced | 2022-09-18: Updated FTP location. |
Publication List
| Dataset with its publication pending |
Keyword List
| submitter keyword: glioblastoma, glioma initiating/stem cell, cancer stem cells, stemness, differentiation |
Contact List
| Norie Araki | |
|---|---|
| lab head | |
| Akiko Nambu | |
| contact affiliation | Kumamoto University |
| dataset submitter | |
Full Dataset Link List
| jPOST dataset URI |
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.jpostdb.org/JPST000355/ |
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