PXD008327

PXD008327 is an original dataset announced via ProteomeXchange.

Title	Kinases orchestrate phospho-proteome dynamics during mitotic exit
Description	The cell division cycle culminates in mitosis when two daughter cells are born. As cyclin-dependent kinase (Cdk) activity reaches its peak, the Anaphase Promoting Complex (APC) is activated to trigger sister chromatid separation and mitotic spindle elongation, followed by spindle disassembly and cytokinesis. Degradation of mitotic cyclins and activation of Cdk-counteracting phosphatases are thought to cause protein dephosphorylation to control these sequential events. Here, we use budding yeast to analyze phosphorylation dynamics of 3456 phosphosites on 1101 proteins with high temporal resolution as cells progress synchronously through mitosis. This illustrates sequential protein dephosphorylation and reveals that the order arises from successive inactivation of S and M phase Cdks and of the mitotic kinase Polo. Unexpectedly, we detect as many new phosphorylation events as there are dephosphorylation events. These correlate with late mitotic kinase activation and identify numerous candidate targets of these kinases. Our findings revise our view of mitotic exit and portray it as a dynamic process in which a range of mitotic kinases instruct the order of both protein dephosphorylation as well as phosphorylation.
HostingRepository	PRIDE
AnnounceDate	2018-10-15
AnnouncementXML	Submission_2018-10-15_12:06:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Andrew Jones
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-29 06:28:00	ID requested
⏵ 1	2018-10-15 12:06:17	announced

Ferreira SA, Motwani MS, Faull PA, Seymour AJ, Yu TTL, Enayati M, Taheem DK, Salzlechner C, Haghighi T, Kania EM, Oommen OP, Ahmed T, Loaiza S, Parzych K, Dazzi F, Varghese OP, Festy F, Grigoriadis AE, Auner HW, Snijders AP, Bozec L, Gentleman E, Bi-directional cell-pericellular matrix interactions direct stem cell fate. Nat Commun, 9(1):4049(2018) [pubmed]

curator keyword: Biological

submitter keyword: Cell cycle, kinases, mitosis, phosphatases, phosphoproteomics

Bram Snijders
contact affiliation	Mass Spectrometry Proteomics Science Technology Platform, The Francis Crick Institute, London, UK
contact email	Bram.Snijders@crick.ac.uk
lab head
Andrew Jones
contact affiliation	The Francis Crick Institute
contact email	Andrew.Jones@crick.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD008327

PRIDE project URI

• PRIDE
  1. PXD008327
     1. Label: PRIDE project
     2. Name: Kinases orchestrate phospho-proteome dynamics during mitotic exit