PXD008323

PXD008323 is an original dataset announced via ProteomeXchange.

Title	Characterization of phosphohistidine phosphatase 1-induced changes in the mouse liver proteome
Description	Phosphohistidine signaling in mammalian biological systems has not been well characterized. In this study, we investigated the impact of phosphohistidine phosphatase 1 (PHPT1) expression on the hepatic proteome profile in vivo. PHPT1 overexpression was carried out using adenoviral-based transgene delivery for liver-specific overexpression of PHPT1 while a heterozygous knockout was used as a model of PHPT1 deficiency. These two models were characterized using mass spectrometry-based proteomics and label-free relative quantitation. In conjunction with bioinformatic analysis, PHPT1-induced changes in several key proteins and pathways were identified in the liver. Implementation of these models in future studies will lead to far greater insight into the role of phosphohistidine signaling and PHPT1 regulation in mammalian cells.
HostingRepository	PRIDE
AnnounceDate	2020-07-29
AnnouncementXML	Submission_2020-07-29_06:41:05.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008323
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Daniel Martin
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-29 05:00:36	ID requested
⏵ 1	2020-07-29 06:41:06	announced

Martin DR, Dutta P, Mahajan S, Varma S, Stevens SM, Structural and activity characterization of human PHPT1 after oxidative modification. Sci Rep, 6():23658(2016) [pubmed]

curator keyword: Biological

submitter keyword: PHPT1, phosphohistidine, hepatic proteomics, mus musculus

Stanley Stevens Jr
contact affiliation	Cell and Molecular Biology Department, at the University of South Florida
contact email	Smstevens@usf.edu
lab head
Daniel Martin
contact affiliation	University of South Florida
contact email	martin32@mail.usf.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD008323

PRIDE project URI

• PRIDE
  1. PXD008323
     1. Label: PRIDE project
     2. Name: Characterization of phosphohistidine phosphatase 1-induced changes in the mouse liver proteome