PXD008323 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Characterization of phosphohistidine phosphatase 1-induced changes in the mouse liver proteome |
Description | Phosphohistidine signaling in mammalian biological systems has not been well characterized. In this study, we investigated the impact of phosphohistidine phosphatase 1 (PHPT1) expression on the hepatic proteome profile in vivo. PHPT1 overexpression was carried out using adenoviral-based transgene delivery for liver-specific overexpression of PHPT1 while a heterozygous knockout was used as a model of PHPT1 deficiency. These two models were characterized using mass spectrometry-based proteomics and label-free relative quantitation. In conjunction with bioinformatic analysis, PHPT1-induced changes in several key proteins and pathways were identified in the liver. Implementation of these models in future studies will lead to far greater insight into the role of phosphohistidine signaling and PHPT1 regulation in mammalian cells. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_05:10:11.243.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD008323 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Daniel Martin |
SpeciesList | scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-11-29 05:00:36 | ID requested | |
1 | 2020-07-29 06:41:06 | announced | |
⏵ 2 | 2024-10-22 05:10:19 | announced | 2024-10-22: Updated project metadata. |
Publication List
Martin DR, Dutta P, Mahajan S, Varma S, Stevens SM, Structural and activity characterization of human PHPT1 after oxidative modification. Sci Rep, 6():23658(2016) [pubmed] |
10.6019/PXD008323; |
10.1038/srep23658; |
Keyword List
curator keyword: Biological |
submitter keyword: mus musculus, phosphohistidine, hepatic proteomics,PHPT1 |
Contact List
Stanley Stevens Jr |
contact affiliation | Cell and Molecular Biology Department, at the University of South Florida |
contact email | Smstevens@usf.edu |
lab head | |
Daniel Martin |
contact affiliation | Cleveland Clinic |
contact email | martin32@mail.usf.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2020/07/PXD008323 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008323
- Label: PRIDE project
- Name: Characterization of phosphohistidine phosphatase 1-induced changes in the mouse liver proteome