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DataSet Summary

  • HostingRepository: PRIDE
  • AnnounceDate: 2018-06-13
  • AnnouncementXML: Submission_2019-02-26_08:17:33.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original data
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: F Butter
  • Title: Tdrd6a regulates the aggregation of Buc into functional subcellular compartments that drive germ cell specification
  • Description: In recent years, it has become clear that membrane-less structures formed through phase separation represent an important class of subcellular compartmentalization. However, little is known about how the formation or disassembly of such compartments is regulated. The Balbiani body (Bb) is a phase-separated structure essential for germ cell specification and home to many germ cell-specific mRNAs and proteins. Throughout oogenesis, the structure assembles all critical components required for germ cell induction upon fertilization, then referred to as germ plasm (Gp). In zebrafish, formation of the Bb requires Bucky ball (Buc), a protein with prion-like properties. We have found that Tdrd6a interacts directly with Buc and regulates its aggregation into a functional Bb. Without Tdrd6a, the morphology and mobility of the Bb and the Gp are severely affected, ultimately resulting in PGC depletion in the progeny. Since germ cells are rich in phase-separated structures, many more of such regulatory interactions exist.
  • SpeciesList: scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
  • ModificationList: phosphorylated residue; dimethylated residue
  • Instrument: Q Exactive

Dataset History

VersionDatetimeStatusChangeLog Entry
02017-11-29 03:30:58ID requested
12018-06-12 23:46:45announced
22019-02-26 08:17:34announcedUpdated publication reference for PubMed record(s): 30086300.

Publication List

  1. Roovers EF, Kaaij LJT, Redl S, Bronkhorst AW, Wiebrands K, de Jesus Domingues AM, Huang HY, Han CT, Riemer S, Dosch R, Salvenmoser W, Grün D, Butter F, van Oudenaarden A, Ketting RF, Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification. Dev Cell, 46(3):285-301.e9(2018) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: LC-MS/MS, peptide pulldown, immunoprecipitation

Contact List

    Falk Butter
    • contact affiliation: Institute of Molecular Biology (IMB) Ackermannweg 4 55128 Mainz
    • contact email: f.butter@imb-mainz.de
    • lab head:
    F Butter
    • contact affiliation: Quantitative Proteomics Institute of Molecular Biology (IMB)
    • contact email: f.butter@imb-mainz.de
    • dataset submitter:

Full Dataset Link List

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Repository Record List

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