PXD008322

PXD008322 is an original dataset announced via ProteomeXchange.

Title	Tdrd6a regulates the aggregation of Buc into functional subcellular compartments that drive germ cell specification
Description	In recent years, it has become clear that membrane-less structures formed through phase separation represent an important class of subcellular compartmentalization. However, little is known about how the formation or disassembly of such compartments is regulated. The Balbiani body (Bb) is a phase-separated structure essential for germ cell specification and home to many germ cell-specific mRNAs and proteins. Throughout oogenesis, the structure assembles all critical components required for germ cell induction upon fertilization, then referred to as germ plasm (Gp). In zebrafish, formation of the Bb requires Bucky ball (Buc), a protein with prion-like properties. We have found that Tdrd6a interacts directly with Buc and regulates its aggregation into a functional Bb. Without Tdrd6a, the morphology and mobility of the Bb and the Gp are severely affected, ultimately resulting in PGC depletion in the progeny. Since germ cells are rich in phase-separated structures, many more of such regulatory interactions exist.
HostingRepository	PRIDE
AnnounceDate	2018-06-13
AnnouncementXML	Submission_2019-02-26_08:17:33.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	F Butter
SpeciesList	scientific name: Danio rerio (Zebrafish) (Brachydanio rerio); NCBI TaxID: 7955;
ModificationList	phosphorylated residue; dimethylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-29 03:30:58	ID requested
1	2018-06-12 23:46:45	announced
⏵ 2	2019-02-26 08:17:34	announced	Updated publication reference for PubMed record(s): 30086300.

Roovers EF, Kaaij LJT, Redl S, Bronkhorst AW, Wiebrands K, de Jesus Domingues AM, Huang HY, Han CT, Riemer S, Dosch R, Salvenmoser W, Gr, ü, n D, Butter F, van Oudenaarden A, Ketting RF, Tdrd6a Regulates the Aggregation of Buc into Functional Subcellular Compartments that Drive Germ Cell Specification. Dev Cell, 46(3):285-301.e9(2018) [pubmed]

curator keyword: Biological

submitter keyword: LC-MS/MS, peptide pulldown, immunoprecipitation

Falk Butter
contact affiliation	Institute of Molecular Biology (IMB) Ackermannweg 4 55128 Mainz
contact email	f.butter@imb-mainz.de
lab head
F Butter
contact affiliation	Quantitative Proteomics Institute of Molecular Biology (IMB)
contact email	f.butter@imb-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008322

PRIDE project URI

• PRIDE
  1. PXD008322
     1. Label: PRIDE project
     2. Name: Tdrd6a regulates the aggregation of Buc into functional subcellular compartments that drive germ cell specification