PXD008318

PXD008318 is an original dataset announced via ProteomeXchange.

Title	ALKBH7, a mitochondrial member of AlkB family, binds histones in vitro, part 1
Description	Human genome encodes 9 AlkB family members, one of which is ALKBH7. In this study, we found recombinant ALKBH7 could bind bulk histones in GST pull-down and attenuate the signal of H4K20me2 in demethylation assays. With mass spectrometry, we also identified several potential ALKBH7-interacting proteins, such as NDUFS2, MRPS7 etc.; and found the expression of NDUFA4 and COX6C was down-regulated upon knockdown of ALKBH7. With these results, we can obtain a novel sight into the function of this protein.
HostingRepository	PRIDE
AnnounceDate	2020-05-26
AnnouncementXML	Submission_2020-05-26_11:36:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wei Ge
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-29 01:59:03	ID requested
⏵ 1	2020-05-26 11:36:40	announced

Meng S, Zhan S, Dou W, Ge W, The interactome and proteomic responses of ALKBH7 in cell lines by in-depth proteomics analysis. Proteome Sci, 17():8(2019) [pubmed]

curator keyword: Biological

submitter keyword: ALKBH7;mitochondrion;histones

Wei Ge
contact affiliation	Institute of Basic Medical Sciences, School of Basic Medicine, Peking Union Medical College; No. 5 Dongdansantiao, Dongcheng District, Beijing 100005, China
contact email	wei.ge@chem.ox.ac.uk
lab head
Wei Ge
contact affiliation	National Key Laboratory of Medical Molecular Biology & Department of Immunology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences
contact email	wei.ge@chem.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008318
     1. Label: PRIDE project
     2. Name: ALKBH7, a mitochondrial member of AlkB family, binds histones in vitro, part 1