PXD008311 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | The impact of oncogenic EGFRvIII on the proteome of extracellular vesicles (exosomes) released from glioblastoma cells |
Description | Glioblastoma multiforme (GBM) is a highly aggressive and heterogeneous form of primary brain tumors, driven by a complex repertoire of oncogenic alterations, including the constitutively active epidermal growth factor receptor (EGFRvIII). EGFRvIII impacts both cell-intrinsic and non-cell autonomous aspects of GBM progression, including cell invasion, angiogenesis and modulation of the tumor microenvoiront, this is, at least in part, attributable to the release and intercellular trafficking of extracellular vesicles (EVs), heterogeneous membrane structures containing multiple bioactive macromolecules. Here we analyzed the impact of EGFRvIII on the profile of glioma EVs using a series of isogenic tumor cell lines, in which this oncogene exhibits a strong transforming activity. Thus, we observed that EGFRvIII expression alters several properties of glioma EVs, including their output and global protein composition. Using mass spectrometry, quantitative proteomic analysis and Gene Ontology terms filters, we observed that EVs released by EGFRVIII-transformed cells were enriched for extracellular exosome and focal adhesion related proteins. Among them, we validated the association of pro-invasive proteins (CD44, BSG, CD151) with EVs of EGFRvIII expressing cells, and down-regulation of exosomal markers (CD81 and CD82) in EVs of their indolent counterparts. Nano-flow cytometry revealed that the EV output from individual glioma cell lines was highly heterogeneous, such that only a fraction of EVs contained specific proteins such as EGFR, CD9, or others. Notably, cells expressing EGFRvIII released ample EVs double positive for CD44/BSG, and these proteins also co-localized in cellular filopodia. We also detected the expression of homophilic adhesion molecules and increased homologous EV uptake by EGFRvIII-positive glioma cells. These results suggest that oncogenic EGFRvIII reprograms the proteome of GBM-related EVs, a notion with considerable implications for their biological activity and properties relevant for development of EV-based cancer biomarkers. |
HostingRepository | PRIDE |
AnnounceDate | 2024-10-22 |
AnnouncementXML | Submission_2024-10-22_04:42:35.141.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD008311 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Dongsic Choi |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Carboxymethyl; Deamidated; Oxidation |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-11-28 01:46:17 | ID requested | |
1 | 2018-07-30 03:03:04 | announced | |
⏵ 2 | 2024-10-22 04:42:40 | announced | 2024-10-22: Updated project metadata. |
Publication List
Choi D, Montermini L, Kim DK, Meehan B, Roth FP, Rak J, The Impact of Oncogenic EGFRvIII on the Proteome of Extracellular Vesicles Released from Glioblastoma Cells. Mol Cell Proteomics, 17(10):1948-1964(2018) [pubmed] |
10.1074/mcp.ra118.000644; |
10.6019/PXD008311; |
Keyword List
submitter keyword: glioblastoma, exosomes, cancer, glioma, extracellular vesicles, heterogeneity,EGFRvIII, nano-flow cytometry, oncogene, invasion |
Contact List
Janusz Rak |
contact affiliation | Janusz Rak MD, PhD Jack Cole Chair in Pediatric Hematology/Oncology Professor, Department of Pediatrics McGill University The Research Institute of the McGill University Health Centre Montreal Children's Hospital 1001 Decarie Blvd, Office # E M1 2244 Montreal, Quebec, H4A 3J1 Phone: (514) 412-4400 ext-office: 76240; -lab: 76220; Fax (514) 412-4331 |
contact email | janusz.rak@mcgill.ca |
lab head | |
Dongsic Choi |
contact affiliation | Research Associate |
contact email | choi.dongsic@mail.mcgill.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/07/PXD008311 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008311
- Label: PRIDE project
- Name: The impact of oncogenic EGFRvIII on the proteome of extracellular vesicles (exosomes) released from glioblastoma cells