PXD008310

PXD008310 is an original dataset announced via ProteomeXchange.

Title	FBXL13 regulates centrosome homeostasis and migration through ubiquitin- mediated proteolysis
Description	Aberrant centrosome organization with ensuing alterations of microtubule nucleation enables tumor cells to proliferate and invade despite increased genomic instability. CEP192 is a key factors in the initiation process of centrosome duplication and in the control of centrosome microtubule nucleation. However, regulatory means of CEP192 have remained unknown. Here we report that FBXL13, a binding determinant of SCF (SKP1-CUL1-F-Box)-family E3 ubiquitin ligases, is enriched at centrosomes and interacts with the centrosomal proteins Centrin-2, Centrin-3, CEP152, and CEP192. Among these, CEP192 is specifically targeted for proteasomal degredation by FBXL13. Accordingly, induced FBXL13 expression downregulates centrosomal γ-tubulin, and disrupts centrosomal microtubule arrays. In addition, depletion of FBXL13 induces high levels of CEP192 and γ-tubulin at the centrosomes corresponding to defects in cell motility. Together, we characterize FBXL13 as a novel regulator of microtubule nucleation activity and highlight a role in promoting cell motility with potential tumor-promoting implications.
HostingRepository	PRIDE
AnnounceDate	2018-01-16
AnnouncementXML	Submission_2018-03-07_23:58:06.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008310
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Roman Fischer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl; Oxidation
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-28 01:43:48	ID requested
1	2018-01-16 06:17:46	announced
⏵ 2	2018-03-07 23:58:07	announced	Updated publication reference for PubMed record(s): 29348145.

Fung E, Richter C, Yang HB, Sch, ä, ffer I, Fischer R, Kessler BM, Bassermann F, D'Angiolella V, FBXL13 directs the proteolysis of CEP192 to regulate centrosome homeostasis and cell migration. EMBO Rep, 19(3):(2018) [pubmed]

curator keyword: Biological

submitter keyword: Ubiquitin FBXl13

Roman Fischer
contact affiliation	Facility Head, Discovery Proteomics Facility University of Oxford
contact email	roman.fischer@ndm.ox.ac.uk
lab head
Roman Fischer
contact affiliation	University of Oxford
contact email	roman.fischer@ndm.ox.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008310
     1. Label: PRIDE project
     2. Name: FBXL13 regulates centrosome homeostasis and migration through ubiquitin- mediated proteolysis