PXD008297

PXD008297 is an original dataset announced via ProteomeXchange.

Title	Proteomics in biomanufacturing control: Protein dynamics of CHO-K1 cells and conditioned media during apoptosis and necrosis
Description	Cell viability has a critical impact on product quantity and quality during the biomanufacturing of therapeutic proteins. An advanced understanding of changes in the cellular and conditioned media proteomes upon cell stress and death is therefore needed for improved bioprocess control. Here, a high pH / low pH reversed phase data independent 2D-LC-MSE discovery proteomics platform was applied to study the cellular and conditioned media proteomes of CHO-K1 apoptosis and necrosis models where cell death was induced by staurosporine exposure or aeration shear in a benchtop bioreactor, respectively. Enriched gene ontology molecular function, biological processes and cellular component terms revealed both cell-death independent and specific features. In addition, label free quantitation using the Hi3 approach resulted in a comprehensive shortlist of 23 potential cell viability marker proteins with highest abundance and a significant increase in the conditioned media upon induction of cell death, including proteins related to cellular stress response, signal mediation, cytoskeletal organization, cell differentiation, cell interaction as well as metabolic and proteolytic enzymes which are interesting candidates for translating into targeted analysis platforms for monitoring bioprocessing response and increasing process control.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:50.690.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Simone Albrecht
SpeciesList	scientific name: Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus); NCBI TaxID: 10029;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue
Instrument	Xevo G2 Q-Tof

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-27 01:35:41	ID requested
1	2018-02-27 04:37:32	announced
⏵ 2	2024-10-22 04:42:59	announced	2024-10-22: Updated project metadata.

10.1002/bit.26563;

Albrecht S, Kaisermayer C, Gallagher C, Farrell A, Lindeberg A, Bones J, Proteomics in biomanufacturing control: Protein dynamics of CHO-K1 cells and conditioned media during apoptosis and necrosis. Biotechnol Bioeng, 115(6):1509-1520(2018) [pubmed]

curator keyword: Technical, Biological

submitter keyword: proteomics, CHO,Keywords: biomanufacturing, necrosis, apoptosis, 2D-LC-MSE

Jonathan Bones
contact affiliation	NIBRT Characterisation and Comparability Laboratory, National Institute for Bioprocessing, Research and Training, Fosters Avenue, Mount Merrion, Blackrock, Co. Dublin, A94 X099, Ireland
contact email	jonathan.bones@nibrt.ie
lab head
Simone Albrecht
contact affiliation	NIBRT
contact email	simone.albrecht@nibrt.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/02/PXD008297

PRIDE project URI

• PRIDE
  1. PXD008297
     1. Label: PRIDE project
     2. Name: Proteomics in biomanufacturing control: Protein dynamics of CHO-K1 cells and conditioned media during apoptosis and necrosis