PXD008296

PXD008296 is an original dataset announced via ProteomeXchange.

Title	Middle-Down Characterization of the Cell Cycle Dependence of Histone H4 PTMs and Proteoforms
Description	In the present study, a middle-down approach is employed for the identification, characterization, and quantitation of the PTMs and proteoforms of histone H4 in two breast cancer cell lines. Our experimental strategy not only preserves the combinatorial attributes of existing PTMs, but also enhances the separation ability and reduces the complexity of downstream data analysis. The fluctuations in the relative abundances of common modifications throughout the cell cycle, including phosphorylation, acetylation, and methylation, suggest that they play significant roles at different stages of the cell cycle in breast cancer and could participate in carcinogenesis. In summary, our analysis advances the understanding and elucidation of cancer epigenetics at the single-molecule level, and lays the foundation for further study of histones or other proteins.
HostingRepository	PRIDE
AnnounceDate	2018-07-05
AnnouncementXML	Submission_2018-07-05_04:58:12.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tingting Jiang
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monomethylated residue; phosphorylated residue; N6: N6; acetylated residue; dimethylated residue
Instrument	Fourier Transform Ion Cyclotron Resonance Mass Spectrometer

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-27 01:30:30	ID requested
⏵ 1	2018-07-05 04:58:14	announced

Jiang T, Hoover ME, Holt MV, Freitas MA, Marshall AG, Young NL, Middle-Down Characterization of the Cell Cycle Dependence of Histone H4 Posttranslational Modifications and Proteoforms. Proteomics, 18(11):e1700442(2018) [pubmed]

curator keyword: Biological

submitter keyword: PTM, ETD, FT-ICR, HILIC, Mass spectrometry

Nicolas L. Young
contact affiliation	Verna & Marrs McLean Department of Biochemistry & Molecular Biology Department of Molecular and Cellular Biology Baylor College of Medicine
contact email	Nicolas.Young@bcm.edu
lab head
Tingting Jiang
contact affiliation	Florida State University
contact email	tingjiang@magnet.fsu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008296
     1. Label: PRIDE project
     2. Name: Middle-Down Characterization of the Cell Cycle Dependence of Histone H4 PTMs and Proteoforms