PXD008283

PXD008283 is an original dataset announced via ProteomeXchange.

Title	Protein Analysis of the Tomato Male Gametophyte Following Heat-stress Exposure and Pre-treatment with an Ethylene-releaser Conferring Pollen Thermotolerance
Description	Heat stress is a major cause for yield loss in many crops, including vegetable crops. Even short waves of high temperature, becoming more frequent during recent years, can be detrimental. Pollen development is most heat-sensitive, being the main cause for reduced productivity under heat-stress across a wide range of crops. The molecular mechanisms involved in pollen heat-stress response and thermotolerance are however not fully understood. Recently, we have demonstrated that ethylene, a gaseous plant hormone, plays a role in tomato (Solanum lycopersicum) pollen thermotolerance. These results were substantiated in the current work showing that increasing ethylene levels by using an ethylene-releasing substance, ethephon, prior to heat-stress exposure, increased pollen quality. A proteomic approach was undertaken, to unravel the mechanisms underlying pollen heat-stress response and ethylene-mediated pollen thermotolerance in developing pollen grains. Proteins were extracted and analyzed by means of a gel LC-MS fractionation protocol, and a total of 1355 proteins were identified. A dataset of 721 proteins, detected in three biological replicates of at least one of the applied treatments, was used for all analyses. Quantitative analysis was performed based on peptide count. The analysis revealed that heat-stress affected the developmental program of pollen, including protein homeostasis (components of the translational and degradation machinery), carbohydrate and energy metabolism. Ethephon-pretreatment shifted the heat-stressed pollen proteome closer to the proteome under non-stressful conditions, namely, by showing higher abundance of proteins involved in protein synthesis, degradation, tricarboxylic acid cycle and RNA regulation. Furthermore, up-regulation of protective mechanisms against oxidative stress was observed following ethephon-treatment (including higher abundance of glutathione-disulfide reductase, glutaredoxin and protein disulfide isomerase). Taken together, the findings identified systemic and fundamental components of pollen thermotolerance, and serve as a valuable quantitative protein database for further research.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:25.438.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Palak Chaturvedi
SpeciesList	scientific name: Solanum lycopersicum; NCBI TaxID: 4081;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap XL

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-24 00:57:27	ID requested
1	2019-11-12 10:17:38	announced
⏵ 2	2024-10-22 04:42:28	announced	2024-10-22: Updated project metadata.

10.3389/fpls.2018.01558;

Jegadeesan S, Chaturvedi P, Ghatak A, Pressman E, Meir S, Faigenboim A, Rutley N, Beery A, Harel A, Weckwerth W, Firon N, Proteomics of Heat-Stress and Ethylene-Mediated Thermotolerance Mechanisms in Tomato Pollen Grains. Front Plant Sci, 9():1558(2018) [pubmed]

curator keyword: Biological

submitter keyword: tomato,Pollen, ethylene, heat stress, Shotgun proteomics

Palak Chaturvedi
contact affiliation	Department of Ecogenomics and Systems Biology, University of Vienna, Austria
contact email	palak.chaturvedi@univie.ac.at
lab head
Palak Chaturvedi
contact affiliation	University of Vienna
contact email	palak.chaturvedi@univie.ac.at
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008283
     1. Label: PRIDE project
     2. Name: Protein Analysis of the Tomato Male Gametophyte Following Heat-stress Exposure and Pre-treatment with an Ethylene-releaser Conferring Pollen Thermotolerance