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PXD008280

PXD008280 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleSuccession of proteomic, transcriptomic and exometabolomic signatures in Alteromonas macleodii degrading a mix of macroalgal polysaccharides
DescriptionPolysaccharides from macroalgae are important bacterial nutrient source and central biogeochemical component in the oceans. To illuminate the cellular mechanisms of polysaccharide degradation by marine bacteria, growth of Alteromonas macleodii 83-1 on a mix of laminarin, alginate and pectin was characterized using transcriptomics, proteomics and exometabolomics. A. macleodii 83-1 showed two distinct growth stages, with exponential growth during laminarin utilization followed by maintenance during simultaneous alginate/pectin utilization. The biphasic growth coincided with major temporal shifts in gene expression and metabolite secretion, enabling to define major/accessory polysaccharide utilization loci, reconstruct the complete degradation pathways for each polysaccharide, as well as identify temporal phenotypes in other relevant traits. FT-ICR-MS revealed a distinct suite of secreted metabolites for each growth phase, with pyrroloquinoline quinone exclusively produced with alginate/pectin. The finding of substrate-unique phenotypes indicates an exquisite adaptation to polysaccharide utilization with probable relevance for the degradation of macroalgal biomass, which comprises a complex mix of polysaccharides. Moreover, substrate-unique exometabolomes possibly influence metabolic interactions with other community members. Overall, the presence of fine-tuned genetic machineries for polysaccharide degradation and the widespread detection of related CAZymes in global locations indicate an ecological relevance of A. macleodii in marine polysaccharide cycling and bacteria-algae interactions.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:42:02.726.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD008280
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAlexandra Dürwald
SpeciesList scientific name: Alteromonas macleodii; NCBI TaxID: 28108;
ModificationListOxidation
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-11-23 07:04:01ID requested
12018-08-22 04:12:31announced
22024-10-22 04:42:03announced2024-10-22: Updated project metadata.
Publication List
10.1038/s41396-018-0252-4;
Koch H, D, ü, rwald A, Schweder T, Noriega-Ortega B, Vidal-Melgosa S, Hehemann JH, Dittmar T, Freese HM, Becher D, Simon M, Wietz M, Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides. ISME J, 13(1):92-103(2019) [pubmed]
10.6019/PXD008280;
Keyword List
curator keyword: Biological
submitter keyword: catabolite repression, polysaccharide degradation,Alteromonas
Contact List
Thomas Schweder
contact affiliationPharmaceutical Biotechnology, Institute of Pharmacy, University of Greifswald, Germany
contact emailschweder@uni-greifswald.de
lab head
Alexandra Dürwald
contact affiliationUni-Greifswald
contact emailduerwalda@uni-greifswald.de
dataset submitter
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Dataset FTP location
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PRIDE project URI
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