PXD008270

PXD008270 is an original dataset announced via ProteomeXchange.

Title	Tyrosine-1 of RNA polymerase II CTD controls global termination of gene transcription in mammals
Description	The aim of the project was to analyse the functional role of Tyrosine-1 of mammalian RNA polymerase II CTD in the regulation of transcription-coupled processes. We studied mammalian CTD mutants to analyze the function of tyrosine1 residues. Mutation of 3/4 of the tyrosine residues in a mutant YFFF resulted in a massive read-through transcription phenotype. Read-through was observed for 3’ processed and polyadenylated RNAs. The YFFF mutant shows a loss of interaction with the Mediator and Integrator complexes in MS analysis.
HostingRepository	PRIDE
AnnounceDate	2018-01-09
AnnouncementXML	Submission_2018-01-09_03:40:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ignasi Forne
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive; LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-23 00:05:25	ID requested
⏵ 1	2018-01-09 03:40:06	announced

Shah N, Maqbool MA, Yahia Y, El Aabidine AZ, Esnault C, Forn, é I, Decker TM, Martin D, Sch, ü, ller R, Krebs S, Blum H, Imhof A, Eick D, Andrau JC, Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals. Mol Cell, 69(1):48-61.e6(2018) [pubmed]

curator keyword: Biological

submitter keyword: RNA Polymerase II, CTD, Tyrosine1, Transcription termination, Read-through transcription, Promoter-proximal pausing, Divergent transcription.

Prof. Dr. Dirk Eick
contact affiliation	Helmholtz Zentrum München German Research Center for Environmental Health (GmbH) Research Unit Molecular Epigenetics Marchioninistraße 25 81377 Munich Tel. +49 89 3187-1512
contact email	eick@helmholtz-muenchen.de
lab head
Ignasi Forne
contact affiliation	Biomedical Center-LMU
contact email	ignasi.forne@lrz.uni-muenchen.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/01/PXD008270

PRIDE project URI

• PRIDE
  1. PXD008270
     1. Label: PRIDE project
     2. Name: Tyrosine-1 of RNA polymerase II CTD controls global termination of gene transcription in mammals