PXD008263

PXD008263 is an original dataset announced via ProteomeXchange.

Title	Induction by Bradyrhizobium diazoefficiens of different catabolic pathways for growth in D-mannitol or L-arabinose leading to pronounced differences in CO2 fixation, O2 consumption, and lateral-flagellum production
Description	Bradyrhizobium diazoefficiens, the soybean N2-fixing symbiont, uses mannitol or arabinose as carbon and energy source, among other substances. In this bacterium, growth with mannitol led to higher biomass production, and inhibited the synthesis of its secondary (lateral) flagellar system, regarding growth with arabinose. A proteomic comparison of bacteria grown in mannitol or arabinose suggested that mannitol may be degraded by the Calvin-Benson-Bassham pathway, while arabinose seems degraded by the L-2-keto-3-deoxyarabonate pathway, both pathways sharing only the reactions from pyruvate to CO2. Despite these differences, the stoichiometric balances resulted in similar O2 consumption and ATP production per C-mole of substrate. The poly-3-hydroxybutyrate biosynthesis pathway was stimulated in mannitol, while in arabinose there was a consistent increase in enzymes for the synthesis of nicotinamide adenine dinucleotide from aspartate, suggesting a surplus of reducing power in mannitol. This may agree to previous reports showing higher O2 consumption rate in arabinose, which taking into account the similarity in O2 consumption balance, suggests higher maintenance energy demand in this carbon and energy source. Since lateral flagella are produced only in arabinose, we wondered whether their energy demand might explain this difference. Hence, we compared the O2 consumption rates in mannitol and arabinose of the wild-type and a lafR::Km mutant devoid of lateral flagella. We observed that, while there was a three-fold higher O2 consumption rate in the wild-type in arabinose with respect to mannitol, there was no difference in the mutant, suggesting that lateral flagella behave as parasitic structures in this medium.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:41:58.257.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD008263
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Carolina Cogo
SpeciesList	scientific name: Bradyrhizobium japonicum USDA 110; NCBI TaxID: 224911;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-22 02:51:13	ID requested
1	2018-06-25 05:47:30	announced
⏵ 2	2024-10-22 04:42:00	announced	2024-10-22: Updated project metadata.

Cogo C, P, é, rez-Gim, é, nez J, Rajeswari CB, Luna MF, Lodeiro AR, Consumption, and Lateral-Flagellum Production. Front Microbiol, 9():1189(2018) [pubmed]

10.3389/fmicb.2018.01189;

10.6019/PXD008263;

curator keyword: Biological

submitter keyword: O2 consumption, L-KDA pathway,CBB pathway, PP pathway, flagella

Anibal Roberto Lodeiro
contact affiliation	Instituto de Biotecnología y Biología Molecular (IBBM). Facultad de Ciencias Exactas-UNLP y CCT La Plata-CONICET
contact email	lodeiro@biol.unlp.edu.ar
lab head
Carolina Cogo
contact affiliation	1-Instituto de Biotecnología y Biología Molecular (IBBM). Facultad de Ciencias Exactas-UNLP y CCT La Plata-CONICET. 2-Departamento de Ciencias Básicas, Facultad de Ingeniería-UNLP
contact email	carolina.cogo@yahoo.com.ar
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008263
     1. Label: PRIDE project
     2. Name: Induction by Bradyrhizobium diazoefficiens of different catabolic pathways for growth in D-mannitol or L-arabinose leading to pronounced differences in CO2 fixation, O2 consumption, and lateral-flagellum production