PXD008240

PXD008240 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mosquito-Derived Anophelin Sulfoproteins are Potent Anti-Thrombotics
Description	we first expressed AAa and AAg in a eukaryotic system to investigate whether the proteins could be sulfated in vivo. For this purpose we used insect cells as a model of the Anopheles mosquito. Specifically, codon-optimized sequences encoding AAa and AAg were designed as N-terminal fusions with the honeybee mellitin signal sequence in order to direct the recombinant proteins to the secretion pathway and were expressed in Trichoplusia ni insect cells. Following expression, the cell medium containing the secreted proteins was analyzed by nanoliquid chromatography coupled to tandem mass spectrometry (nanoLC-MS/MS).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:42:02.580.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Benjamin Parker
SpeciesList	scientific name: Anopheles albimanus; NCBI TaxID: 7167; scientific name: Anopheles gambiae (African malaria mosquito); NCBI TaxID: 7165;
ModificationList	sulfated residue; deamidated residue
Instrument	Orbitrap Fusion ETD

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-20 05:38:27	ID requested
1	2018-08-30 10:10:47	announced
⏵ 2	2024-10-22 04:42:09	announced	2024-10-22: Updated project metadata.

Publication List

10.1021/acscentsci.7b00612;
Watson EE, Liu X, Thompson RE, Ripoll-Rozada J, Wu M, Alwis I, Gori A, Loh CT, Parker BL, Otting G, Jackson S, Pereira PJB, Payne RJ, Mosquito-Derived Anophelin Sulfoproteins Are Potent Antithrombotics. ACS Cent Sci, 4(4):468-476(2018) [pubmed]

10.1021/acscentsci.7b00612;

Watson EE, Liu X, Thompson RE, Ripoll-Rozada J, Wu M, Alwis I, Gori A, Loh CT, Parker BL, Otting G, Jackson S, Pereira PJB, Payne RJ, Mosquito-Derived Anophelin Sulfoproteins Are Potent Antithrombotics. ACS Cent Sci, 4(4):468-476(2018) [pubmed]

Keyword List

curator keyword: Biological, Biomedical
submitter keyword: protein tyrosine sulfation, anophelin

curator keyword: Biological, Biomedical

submitter keyword: protein tyrosine sulfation, anophelin

Contact List

Benjamin Parker
contact affiliation	The University of Sydney
contact email	benjamin.parker@sydney.edu.au
lab head
Benjamin Parker
contact affiliation	The University of Melbourne
contact email	ben.parker@unimelb.edu.au
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD008240
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD008240

PRIDE project URI

Repository Record List

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