PXD008234

PXD008234 is an original dataset announced via ProteomeXchange.

Title	Absolute Quantitation of All Plasma Proteins from SWATH Data for Biomarker Discovery
Description	The current state of proteomics requires a choice between targeted and discovery methods. The former provides exceptional quantitation and data completeness, but only with few analytes. Discovery proteomics can identify hundreds and thousands of proteins in a sample, but with poor quantitation and data completeness. We have established and optimized a method that combines targeted and data-independent acquisition for absolute quantitation of all plasma proteins in a single sequential window acquisition of all theoretical fragment ions (SWATH) acquisition run using a panel of spike-in standards (SIS). We compared the absolute quantitation (AQ) of SWATH and high-resolution multiple-reaction monitoring (MRM-HR) acquisition methods using the 100 protein PlasmaDive SIS panel spiked into human plasma. SWATH provided equivalent quantitation and differentially abundant protein profiles as MRM-HR. Absolute quantities of the SIS peptides from the SWATH data were used to estimate the absolute quantities (eAQ) for all the proteins in the run. The eAQ values provided similar quantitation and differentially abundant protein profiles as AQ and protein group (PG) values, and the eAQ method was the only scheme where all selected proteins were verified by MRM-HR. We applied the eAQ method to a cohort of 16 non-small cell lung cancer (NSCLC) patients receiving immunotherapeutics and found that fibronectin (FN1) and proteoglycan 4 (PRG4) were useful markers for predicting patients who would stay on these agents for at least 6 months (area under the curve of 0.8438 and 0.6735, respectively). Thirteen additional plasma samples confirmed that FN1 and PRG4 are putative biomarkers (positive predictive value is 100% and 85.7%, respectively) for a prolonged (>6 months) response to immunotherapeutic agents. In conclusion, we describe an optimized method for absolute quantification of all proteins in a SWATH run, and this approach is amenable for the identification of plasma biomarkers.
HostingRepository	PRIDE
AnnounceDate	2019-01-02
AnnouncementXML	Submission_2019-01-02_05:31:56.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Shawn Rice
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; carboxylated residue; monohydroxylated residue; acetylated residue; deamidated residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-17 08:53:12	ID requested
⏵ 1	2019-01-02 05:31:58	announced

Rice SJ, Liu X, Zhang J, Belani CP, Absolute Quantification of All Identified Plasma Proteins from SWATH Data for Biomarker Discovery. Proteomics, 19(3):e1800135(2019) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: SWATH, data-independent acquisition, absolute quantification, plasma biomarkers, lung cancer

Chandra P. Belani
contact affiliation	Department of Medicine, Penn State Cancer Institute, Penn State College of Medicine, Hershey, PA.
contact email	cbelani@pennstatehealth.psu.edu
lab head
Shawn Rice
contact affiliation	Penn State College of Medicine
contact email	srice@pennstatehealth.psu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008234
     1. Label: PRIDE project
     2. Name: Absolute Quantitation of All Plasma Proteins from SWATH Data for Biomarker Discovery