PXD008223

PXD008223 is an original dataset announced via ProteomeXchange.

Title	CX-MS of TFIIH and Def1 complex
Description	The DNA damage response is an essential process for the survival of living cells. In a subset of stress-responsive genes in humans,Elongin controls transcription in response to multiple stimuli, such as DNA damage, oxidative stress, and heat shock. Yeast Elongin (Ela1-Elc1), along with Def1, is known to facilitate ubiquitylation and degradation of RNA polymerase II (pol II) in response tomultiple stimuli, yet transcription activity has not been examined. We have found that Def1 copurifies from yeast whole-cell extract with TFIIH,the largest general transcription factor required for transcription initiation and nucleotide excision repair. The addition of recombinant Def1 and Ela1-Elc1 enhanced transcription initiation in an in vitro reconstituted system including pol II, the general transcription factors, and TFIIS. Def1 also enhanced transcription restart from TFIIS-induced cleavage in a pol II transcribing complex. In the Δdef1 strain, heat shock genes were misregulated, indicating that Def1 is required for induction of some stress-responsive genes in yeast. Taken together, our results extend the understanding of the molecular mechanism of transcription regulation on cellular stress and reveal functional similarities to the mammalian system.
HostingRepository	PRIDE
AnnounceDate	2018-10-22
AnnouncementXML	Submission_2018-10-22_10:21:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nir Kalisman
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-16 01:20:32	ID requested
⏵ 1	2018-10-22 10:21:25	announced

Damodaren N, Van Eeuwen T, Zamel J, Lin-Shiao E, Kalisman N, Murakami K, Def1 interacts with TFIIH and modulates RNA polymerase II transcription. Proc Natl Acad Sci U S A, 114(50):13230-13235(2017) [pubmed]

curator keyword: Biological

submitter keyword: transcription, RNA polymerase II, TFIIH,stress response, Def1

Nir Kalisman
contact affiliation	Dept. of Biological Chemistry, The Hebrew University of Jerusalem
contact email	nirka@mail.huji.ac.il
lab head
Nir Kalisman
contact affiliation	Dept. of Biological Chemistry, The Hebrew University of Jerusalem
contact email	nirka@mail.huji.ac.il
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD008223
     1. Label: PRIDE project
     2. Name: CX-MS of TFIIH and Def1 complex