PXD008219

PXD008219 is an original dataset announced via ProteomeXchange.

Title	Plasmodium falciparum Kelch13, Eps15 and Clathrin HC interactomes obtained using a dimerisation-induced quantitative BioID (DiQ-BioID)
Description	In order to identify Kelch13 interaction partners in living P. falciparum parasites, we established a new type of BioID we termed dimerisation-induced quantitative BioID (DiQ-BioID) that takes advantage of the FKBP-FRB heterodimerisation system. For DiQ-BioID the target is endogenously tagged with FKBP. The promiscuous biotin ligase BirA* is fused with FRB and expressed in the cells expressing the FKBP tagged target. Upon addition of the dimerising ligand (rapalog) BirA*-FRB is recruited onto the FKBP fused target. In the presence of biotin this results in the biotinylation of the target and its interactors whereas in the control the cellular background is biotinylated. As matched parasite cultures (identical cultures grown with and without rapalog) are used, quantitative mass spectrometry was employed to identify specific interaction partners (or compartment neighbors). This was done for Kelch13 as a bait, for one of the so identified hits (Eps15) in reciprocal DiQ-BioID experiments and for the clathrin heavy chain as a negative control (a protein that like Kelch13 and Eps15 is found in foci in the cells but does not co-localise with these targets).
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:36.348.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wieteke Hoeijmakers
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationList	L-cysteine methyl disulfide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-15 07:59:13	ID requested
1	2021-12-02 07:21:39	announced
⏵ 2	2024-10-22 05:30:38	announced	2024-10-22: Updated project metadata.

10.1126/science.aax4735;

Birnbaum J, Scharf S, Schmidt S, Jonscher E, Hoeijmakers WAM, Flemming S, Toenhake CG, Schmitt M, Sabitzki R, Bergmann B, Fr, ö, hlke U, Mes, é, n-Ram, í, rez P, Blancke Soares A, Herrmann H, B, á, rtfai R, Spielmann T, A Kelch13-defined endocytosis pathway mediates artemisinin resistance in malaria parasites. Science, 367(6473):51-59(2020) [pubmed]

curator keyword: Biological

submitter keyword: Eps15, dimerisation-induced quantitative BioID (DiQ-BioID),Plasmodium falciparum, Artemisinin resistance, Vesicle trafficking, Kelch13, Clathrin HC

Tobias Spielmann
contact affiliation	Parasitology Section Bernhard Nocht Institute for Tropical Medicine Bernhard-Nocht-Str. 74 20359 Hamburg Germany
contact email	spielmann@bni-hamburg.de
lab head
Wieteke Hoeijmakers
contact affiliation	Department of Molecular Biology, Faculty of Science, Radboud University, Nijmegen The Netherlands
contact email	w.hoeijmakers@ncmls.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/12/PXD008219

PRIDE project URI

• PRIDE
  1. PXD008219
     1. Label: PRIDE project
     2. Name: Plasmodium falciparum Kelch13, Eps15 and Clathrin HC interactomes obtained using a dimerisation-induced quantitative BioID (DiQ-BioID)