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PXD008215

PXD008215 is an original dataset announced via ProteomeXchange.

Dataset Summary

  • Title: Aurora B kinase disrupts the extended conformation of lattice-bound Kinesin-13 MCAK
  • Description: We reveal that MCAK has a compact conformation in solution using cross-linking and electron microscopy. When MCAK is bound to the microtubule ends, it adopts an extended conformation with the N terminus and neck region of MCAK interacting with the microtubule. Also Aurora Bphosphorylation does not alter MCAK conformation in solution. Aurora B interferes with the extended conformation of MCAK on microtubules to decrease the affinity of MCAK for microtubules and reduces its depolymerase activity in a graded fashion.
  • HostingRepository: PRIDE
  • AnnounceDate: 2019-01-07
  • AnnouncementXML: Submission_2019-01-07_23:48:40.xml
  • DigitalObjectIdentifier:
  • ReviewLevel: Peer-reviewed dataset
  • DatasetOrigin: Original dataset
  • RepositorySupport: Unsupported dataset by repository
  • PrimarySubmitter: Juan Zou
  • SpeciesList: scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
  • ModificationList: phosphorylated residue
  • Instrument: LTQ Orbitrap Velos

Dataset History

RevisionDatetimeStatusChangeLog Entry
02017-11-15 01:32:25ID requested
12019-01-07 09:38:49announced
22019-01-07 23:48:41announcedUpdated publication reference for PubMed record(s): 30578316.

Publication List

  1. McHugh T, Zou J, Volkov VA, Bertin A, Talapatra SK, Rappsilber J, Dogterom M, Welburn JPI, The depolymerase activity of MCAK shows a graded response to Aurora B kinase phosphorylation through allosteric regulation. J Cell Sci, 132(4):(2019) [pubmed]

Keyword List

  1. curator keyword: Biological
  2. submitter keyword: MCAK, Aurora B, microtubules, phosphorylation

Contact List

    Prof. Juri Rappsilber
    • contact affiliation: Wellcome Trust Centre for Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, Scotland, UK Chair of Bioanalytics, Institute of Biotechnology, Technische Universitšt Berlin, Berlin, German
    • contact email: juri.rappsilber@ed.ac.uk
    • lab head:
    Juan Zou
    • contact affiliation: The University of Edinburgh
    • contact email: jzou@ed.ac.uk
    • dataset submitter:

Full Dataset Link List

  1. Dataset FTP location
  2. PRIDE project URI
Repository Record List
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