PXD008208

PXD008208 is an original dataset announced via ProteomeXchange.

Title	Plasmodium falciparum MSRP6-C-terminal domain (cd) interactome vs Maurer’s Cleft proteome and host cytosolic proteome using quantitative BioID
Description	Interaction partners to a small part of the Maurer's clefts protein MSRP6 termed 'cd' were identified using BioID. The cd part recruits MSRP6 to the Maurer's clefts. To find the interactors of cd in isolation from MSRP6, this region was appended together with BirA* (for BioID) to a truncated version of REX3 to traffic it to the host cell (construct REX3trunc-3cd-BirA*-GFP). This construct is found at the Maurer's clefts by virtue of the cd domain. To obtain a BioID proteome specific for cd, two control constructs were generated. First, REX3trunc-BirA*-GFP without cd (freely soluble in the host cell providing a host cytosolic proteome) and STEVOR1-260-BirA*-GFP (as a control for general Maurer's clefts proteins proving a Maurer’s Cleft proteome). Quantitative mass spectrometry was then used to identify candidates for cd-specific MSRP6 interaction partners compared to general Maurer's clefts proteins and exported proteins not localizing at the Maurer's clefts.
HostingRepository	PRIDE
AnnounceDate	2022-03-21
AnnouncementXML	Submission_2022-03-21_07:42:57.766.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Wieteke Hoeijmakers
SpeciesList	scientific name: Plasmodium falciparum; NCBI TaxID: 5833;
ModificationList	L-cysteine methyl disulfide
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-14 02:03:47	ID requested
⏵ 1	2022-03-21 07:42:58	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: Plasmodium falciparum, protein export, Pexel negative exported proteins (PNEPs), MRSP6, Maurer’s Clefts, quantitative BioID

Tobias Spielmann
contact affiliation	Parasitology Section Bernhard Nocht Institute for Tropical Medicine Bernhard-Nocht-Str. 74 20359 Hamburg Germany
contact email	spielmann@bni-hamburg.de
lab head
Wieteke Hoeijmakers
contact affiliation	Department of Molecular Biology, Faculty of Science, Radboud University, Nijmegen The Netherlands
contact email	w.hoeijmakers@ncmls.ru.nl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/03/PXD008208

PRIDE project URI

• PRIDE
  1. PXD008208
     1. Label: PRIDE project
     2. Name: Plasmodium falciparum MSRP6-C-terminal domain (cd) interactome vs Maurer’s Cleft proteome and host cytosolic proteome using quantitative BioID