PXD008197

PXD008197 is an original dataset announced via ProteomeXchange.

Title	Spliceosome specifically associates with CTD phospho-serine 5 isoform of RNA polymerase II to promote co-transcriptional splicing
Description	Co-transcriptional splicing of introns is a defining feature of eukaryotic gene expression. We show that the mammalian spliceosome specifically associates with the S5P CTD isoform of RNA polymerase II (Pol II) as it elongates across spliced exons of protein coding genes, both in human Hela and murine lymphoid cell lines. Immuno-precipitation of MNase digested chromatin with phospho CTD specific antibodies reveals that components of the active spliceosome (both snRNA and proteins) form a specific complex with S5P CTD Pol II. Furthermore a dominant splicing intermediate formed by cleavage at intron 5’ss results in the tethering of upstream exons to this complex at all spliced exons. These are invariably connected to upstream spliced constitutive and less frequently to alternative exons. Finally S5P CTD Pol II accumulates over spliced exons but not adjacent introns. We propose that mammalian splicing employs a rapid, co-transcriptional splicing mechanism based on CTD phosphorylation transitions.
HostingRepository	PRIDE
AnnounceDate	2018-10-05
AnnouncementXML	Submission_2018-10-05_07:40:35.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Kenny Rebelo
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ XL

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-13 08:02:49	ID requested
⏵ 1	2018-10-05 07:40:35	announced

Dataset with its publication pending

curator keyword: Biological

submitter keyword: net-seq, transcription, splicing, spliceosome, CTD

Maria Carmo Fonseca
contact affiliation	Instituto de Medicina Molecular
contact email	carmo.fonseca@medicina.ulisboa.pt
lab head
Kenny Rebelo
contact affiliation	Instituto de Medicina Molecular - MC-Fonseca Lab
contact email	kennyrebelo@medicina.ulisboa.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD008197

PRIDE project URI

• PRIDE
  1. PXD008197
     1. Label: PRIDE project
     2. Name: Spliceosome specifically associates with CTD phospho-serine 5 isoform of RNA polymerase II to promote co-transcriptional splicing