PXD008196

PXD008196 is an original dataset announced via ProteomeXchange.

Title	The acute host – pathogen interactions between Aspergillus fumigatus and Galleria mellonella
Description	Larvae of the greater wax moth Galleria mellonella are an ideal in vivo model to quickly and easily assess the virulence of a range of human pathogens, to comprehensively analyse the host – pathogen interactome and to reproducibly evaluate the in vivo toxicity and efficacy of anti-microbial agents. G. mellonella larvae are easy to inoculate, generate results within 48 hours and are free from the ethical and legal restrictions which surround the use of mammals for this type of testing.1,2 Microbial virulence and pathogenesis can be assessed by a variety of endpoints including survival, fluctuations in hemocyte density, oscillations in fungal burden and changes in hemolymph proteome. Insect hemocytes can be easily isolated and used in ex vivo cellular assays to determine phagocyte – pathogen interactions. Aspergillus fumigatus infection and dissimiation as well as G. mellonella cellular and humoral immune responses were analysed over 24 hours.
HostingRepository	PRIDE
AnnounceDate	2018-06-21
AnnouncementXML	Submission_2018-07-04_05:55:13.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Gerard Sheehan
SpeciesList	scientific name: Galleria mellonella; NCBI TaxID: 7137;
ModificationList	iodoacetic acid derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-13 07:59:16	ID requested
1	2018-06-21 03:14:45	announced
2	2018-06-21 03:33:04	announced	Updated project metadata.
⏵ 3	2018-07-04 05:55:15	announced	Updated project metadata.

Sheehan G, Clarke G, Kavanagh K, Characterisation of the cellular and proteomic response of Galleria mellonella larvae to the development of invasive aspergillosis. BMC Microbiol, 18(1):63(2018) [pubmed]

curator keyword: Biological

submitter keyword: Galleria Aspergillus virulence infection mini-model

Kevin Kavanagh
contact affiliation	Medical Mycology Unit, Department of Biology, Maynooth University, Maynooth, Co. Kildare
contact email	kevin.kavanagh@mu.ie
lab head
Gerard Sheehan
contact affiliation	Maynooth University
contact email	gerard.sheehan.2013@mumail.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/06/PXD008196

PRIDE project URI

• PRIDE
  1. PXD008196
     1. Label: PRIDE project
     2. Name: The acute host – pathogen interactions between Aspergillus fumigatus and Galleria mellonella