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PXD008193

PXD008193 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleA method for producing heat-stable and pure trypsin
DescriptionIn this study we evaluated several different strategies for reductive methylation and purification of porcine trypsin in order to write a protocol that can be followed by proteomics laboratories.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:42:06.152.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterSøren Heissel
SpeciesList scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; scientific name: Equus caballus (Horse); NCBI TaxID: 9796;
ModificationListmonohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-11-13 01:51:14ID requested
12019-06-06 05:41:54announced
22019-07-09 04:49:28announcedUpdated publication reference for PubMed record(s): 31246970.
32024-10-22 04:42:10announced2024-10-22: Updated project metadata.
Publication List
10.1371/journal.pone.0218374;
Heissel S, Frederiksen SJ, Bunkenborg J, H, ø, jrup P, Enhanced trypsin on a budget: Stabilization, purification and high-temperature application of inexpensive commercial trypsin for proteomics applications. PLoS One, 14(6):e0218374(2019) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: Trypsin, Dimethylation, LC-MS
Contact List
Peter Højrup
contact affiliationDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense M, DK
contact emailphp@bmb.sdu.dk
lab head
Søren Heissel
contact affiliationUniversity of Southern Denmark
contact emailsheissel@bmb.sdu.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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