PXD008190

PXD008190 is an original dataset announced via ProteomeXchange.

Title	Comparison of Protein Expressions of Drug-Metabolizing Enzymes between Human Liver and the Hepatic Cell Lines HepG2, Hep3B and Huh7 using SWATH and MRM-HR Proteomics
Description	Human hepatic cell lines have been widely used as an in vitro model for the study of drug metabolism and liver toxicity. However, the validity of this model is still a subject of debate because the expressions of various proteins including drug-metabolizing enzymes (DMEs) in the cell lines can differ significantly from that of human livers. In the present study, we first conducted an untargeted proteomics of the microsomes of the cell lines HepG2, Hep3B, and Huh7 in comparison with human livers using a SWATH method. Furthermore, a targeted proteomic approach, named high-resolution multiple reaction monitoring (MRM-HR), was utilized to compare the expressions of pre-selected DMEs between human livers and the cell lines.
HostingRepository	PRIDE
AnnounceDate	2018-04-18
AnnouncementXML	Submission_2018-04-18_02:11:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jian Shi
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-13 01:38:21	ID requested
⏵ 1	2018-04-18 02:11:28	announced

Shi J, Wang X, Lyu L, Jiang H, Zhu HJ, Comparison of protein expression between human livers and the hepatic cell lines HepG2, Hep3B, and Huh7 using SWATH and MRM-HR proteomics: Focusing on drug-metabolizing enzymes. Drug Metab Pharmacokinet, 33(2):133-140(2018) [pubmed]

curator keyword: Biomedical

submitter keyword: Drug-metabolizing Enzymes, Hep3B, HepG2, Huh7, Human Liver, MRM-HR, Protein Expression, Proteomics, SWATH

Hao-Jie Zhu
contact affiliation	Department of Clinical Pharmacy University of Michigan College of Pharmacy Ann Arbor, MI
contact email	hjzhu@med.umich.edu
lab head
Jian Shi
contact affiliation	University of Michigan
contact email	jianshi@umich.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/04/PXD008190

PRIDE project URI

• PRIDE
  1. PXD008190
     1. Label: PRIDE project
     2. Name: Comparison of Protein Expressions of Drug-Metabolizing Enzymes between Human Liver and the Hepatic Cell Lines HepG2, Hep3B and Huh7 using SWATH and MRM-HR Proteomics