PXD008187

PXD008187 is an original dataset announced via ProteomeXchange.

Title	Proteomic characterization of fresh spermatozoa and supernatant after cryopreservation in relation to freezability of carp (Cyprinus carpio) semen
Description	Our recent studies suggested that the freezability of carp semen is related to seminal plasma protein profiles. Here, we aimed to compare the spermatozoa proteomes of good (GF) and poor (PF) freezability semen of carp. To achieve this, we used two-dimensional difference in gel electrophoresis followed by MALDI-TOF/TOF mass spectrometry. The semen was classified as GF or PF based on sperm motility after freeze/thawing. We identified proteins enriched in spermatozoa of GF (22 proteins) and PF (18 proteins) semen. We also identified 12 proteins enriched in the supernatant after cryopreservation of PF semen. Good freezability is related to high concentrations of proteins involved in the maintenance of flagella structure, membrane fluidity, efficient control of Ca2+ and sperm motility, energy production, and antioxidative protection, which likely reflects the full maturation status of spermatozoa of GF semen. On the other hand poor freezability seems to be related to the presence of proteins identified as released in high quantities from cryopreserved sperm of PF. Thus, the identified proteins might be useful bioindicators of freezing resilience and could be used to screen carp males before cryopreservation, thus improve long-term sperm preservation in carp.
HostingRepository	PRIDE
AnnounceDate	2018-03-23
AnnouncementXML	Submission_2018-03-23_05:40:08.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Mariola Dietrich
SpeciesList	scientific name: Cyprinus carpio (Common carp); NCBI TaxID: 7962;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	autoflex

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-10 06:37:32	ID requested
⏵ 1	2018-03-23 05:40:09	announced

Dietrich MA, Ciereszko A, Proteomic characterization of fresh spermatozoa and supernatant after cryopreservation in relation to freezability of carp (Cyprinus carpio L) semen. PLoS One, 13(3):e0192972(2018) [pubmed]

curator keyword: Biological

submitter keyword: Cryoresilience, cryopreservation, fish, proteome, semen, sperm

Mariola Dietrich
contact affiliation	Institute of Animal Reproduction and Food Research of Polish Academy of Sciences
contact email	m.dietrich@pan.olsztyn.pl
lab head
Mariola Dietrich
contact affiliation	Polish Academy of Sciences
contact email	m.dietrich@pan.olsztyn.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/03/PXD008187

PRIDE project URI

• PRIDE
  1. PXD008187
     1. Label: PRIDE project
     2. Name: Proteomic characterization of fresh spermatozoa and supernatant after cryopreservation in relation to freezability of carp (Cyprinus carpio) semen