PXD008183 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Human Ovarian Proteome |
Description | Fertility preservation research in women today is increasingly taking advantage of bioengineering techniques to develop new biomimetic materials and solutions to safeguard ovarian cell function and microenvironment in vitro and in vivo. However, available data on the human ovary are limited and fundamental differences between animal models and humans are hampering researchers in their quest for more extensive knowledge of human ovarian physiology and key reproductive proteins that need to be preserved. We therefore turned to multi-dimensional label-free mass spectrometry to analyze human ovarian cortex, as it is a high-throughput and conclusive technique providing information on the proteomic composition of complex tissues like the ovary. In-depth proteomic profiling through two-dimensional liquid chromatography-mass spectrometry, western blot, histological and immunohistochemical analyses, and data mining helped us to confidently identify 1,508 proteins. Moreover, our method allowed us to chart the most complete representation so far of the ovarian matrisome, defined as the ensemble of extracellular matrix proteins and associated factors, including more than 80 proteins. In conclusion, this study will provide a better understanding of ovarian proteomics, with a detailed characterization of the ovarian follicle microenvironment, in order to enable bioengineers to create biomimetic scaffolds for transplantation and three-dimensional in vitro culture. By publishing our proteomic data, we also hope to contribute to accelerating biomedical research into ovarian health and disease in general |
HostingRepository | PRIDE |
AnnounceDate | 2018-02-27 |
AnnouncementXML | Submission_2018-02-27_10:25:45.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Didier Vertommen |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2017-11-10 05:36:30 | ID requested | |
⏵ 1 | 2018-02-27 10:25:46 | announced | |
Publication List
Ouni E, Vertommen D, Chiti MC, Dolmans MM, Amorim CA, A Draft Map of the Human Ovarian Proteome for Tissue Engineering and Clinical Applications. Mol Cell Proteomics, 18(Suppl 1):S159-S173(2019) [pubmed] |
Keyword List
curator keyword: Biological, Biomedical |
submitter keyword: Ovarian, Matrisome, proteome |
Contact List
Christiani Andrade Amorim |
contact affiliation | Université catholique de Louvain Institut de recherche expérimentale et clinique (IREC) GYNE Avenue Mounier 52/B1.52.02 1200 Woluwe-Saint-Lambert Belgium |
contact email | christiani.amorim@uclouvain.be |
lab head | |
Didier Vertommen |
contact affiliation | UCL - de Duve Institute, Brussels Belgium |
contact email | didier.vertommen@uclouvain.be |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD008183
- Label: PRIDE project
- Name: Human Ovarian Proteome