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PXD008182

PXD008182 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAdaptation of CysPAT technique to characterize endogenous S-nitrosylation in RAW264.7 cells
DescriptionThe aim of the study was to adapt the CysPAT technique to efficiently identify and characterize endogenous S-nitrosylation in cells under physiological conditions of an experiment from a small amount of sample. Using a macrophage cell line RAW 264.7 we compared the efficacy of the method in the identification of total endogenous S-nitrosylation and the corresponding modification of the samples treated with exogenous SNO donor (GSNO) or with DTT. We identified 999 unique formerly S-nitrosylated peptides (SIA peptides) and 965 unique SNO sites from untreated RAW cells which belong to 569 endogenous nitrosylated proteins. We discovered 579 novel S-nitrosylation sites and identified 232 proteins as new S-nitrosylation targets. A total of 1450 S-nitrosylated peptides belonging to 795 proteins were identified in samples treated with GSNO (n = 3). Interestingly, we found a large overlap (>53%) of S-nitrosylated peptides in both subsets of samples, demonstrating a high sensitivity of the method to analyze endogenous S-nitrosylation. The large number of identified endogenous S-nitrosylated peptides allowed the identification of two nitrosylation consensus sites, and to highlight protein translation and redox processes as key S-nitrosylation targets in macrophages.
HostingRepositoryPRIDE
AnnounceDate2022-02-22
AnnouncementXMLSubmission_2022-02-22_03:46:47.359.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterMaria Ibañez-Vea
SpeciesList scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationListN-ethylmaleimide derivatized cysteine; phosphorylated residue; iodoacetamide derivatized residue
InstrumentTripleTOF 5600
Dataset History
RevisionDatetimeStatusChangeLog Entry
02017-11-10 05:36:02ID requested
12022-02-22 03:46:48announced
Publication List
Ib, á, ñ, ez-Vea M, Huang H, Mart, í, nez de Morentin X, P, é, rez E, Gato M, Zuazo M, Arasanz H, Fern, á, ndez-Irigoyen J, Santamar, í, a E, Fernandez-Hinojal G, Larsen MR, Escors D, Kochan G, Chromatography. J Proteome Res, 17(3):1172-1182(2018) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Proteomics, post-translational modifications (PTMs), S-nitrosylation, macrophages, immune system
Contact List
David Escors
contact affiliationImmunomodulation Group, Navarrabiomed Biomedical Research Center, Institute of Health Carlos III (ISCIII), Navarra Institute for Health Research (IdiSNA), Spain
contact emaildavid.escors.murugarren@navarra.es
lab head
Maria Ibañez-Vea
contact affiliationNavarrabiomed Biomedical Research Center
contact emailmaria.ibanez.vea@navarra.es
dataset submitter
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