PXD008178

PXD008178 is an original dataset announced via ProteomeXchange.

Title	Substrates of human TRAP complex
Description	While the Sec61-complex in the membrane of the human endoplasmic reticulum facilitates translocation of all precursor polypeptides with amino-terminal signal peptides or transmembrane helices, the Sec61-associated translocon-associated protein (TRAP)-complex supports translocation of only a subset of precursor polypeptides, i.e. in a substrate-specific manner. To characterize TRAP-dependent precursors, we combined siRNA-mediated TRAP depletion in HeLa cells, label-free quantitative proteomics, and differential expression analysis. Sec61 served as a positive control
HostingRepository	PRIDE
AnnounceDate	2018-08-09
AnnouncementXML	Submission_2018-09-17_00:11:50.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nagarjuna Nagaraj
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2017-11-09 06:17:55	ID requested
1	2018-08-09 09:20:00	announced
⏵ 2	2018-09-17 00:11:52	announced	Updated publication reference for PubMed record(s): 30217974.

Nguyen D, Stutz R, Schorr S, Lang S, Pfeffer S, Freeze HH, F, ö, rster F, Helms V, Dudek J, Zimmermann R, Proteomics reveals signal peptide features determining the client specificity in human TRAP-dependent ER protein import. Nat Commun, 9(1):3765(2018) [pubmed]

curator keyword: Biological

submitter keyword: HeLa cells, ER protein import, Sec61 complex, TRAP complex, MS

Prof. Dr. Richard Zimmermann
contact affiliation	Medizinische Biochemie und Molekularbiologie Universität des Saarlandes Gebäude 44 Kirrbergerstr. 100 66421 Homburg Deutschland phone: +49 (0)6841 1626510 fax: +49 (0)6841 1626288
contact email	richard.zimmermann@uks.eu
lab head
Nagarjuna Nagaraj
contact affiliation	MaxPlanck Institute for Biochemistry, Core facility
contact email	nagaraj@biochem.mpg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/08/PXD008178

PRIDE project URI

• PRIDE
  1. PXD008178
     1. Label: PRIDE project
     2. Name: Substrates of human TRAP complex